SERT Antibody 340 004 from Synaptic Systems GmbH

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Acta neuropathologica communications
January 15 2019

Fig 1: Experimental design and model validation. a Experimental timeline showing LID-inducing L-DOPA paradigm, motor behavior evaluations, and DA agonist treatments. AIM score ratings were taken at each injection where indicated. b and c Representative TH immunoreactivity in the striatum (b) and substantia nigra (c) showing complete loss of TH-positive neurons and projections following 6-OHDA lesions (scale bar = 1 mm). d Total enumeration of remaining TH neurons in the substantia nigra. e and f IHC for the D2 receptor (e) or GFP (f) in the DRN, showing successful targeting of the structure and robust expression of the transgene (scale bar = 1 mm). Cell bodies were efficiently transduced in the DRN (e and f, bottom insets, scale bar = 50um) and could be seen filling projection fibers in the peduncles (e and f, top insets, scale bar = 50um). k-p Dual labeling transgene expression and SERT in rAAV-D2Rs k-m and rAAV-GFP (n-p) animals. Transgene expression was visualized with D2Rs (l) or GFP (o) staining, and serotonin fiber and cell integrity were confirmed by staining for SERT (m and p). No adverse effects on SERT fibers were observed following vector transduction with either construct (k and n) (k-p scale bar = 100um)
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Cell
September 01 2022

Fig 2: 5-HTR6-primary cilia are in contact with serotonergic axonal varicosities(A) HTR6 (labeled by CF633, green in merged panel) is highly enriched in CA1 neuronal primary cilia (ADCY3, magenta in the merged panel: 20 µm MIP).(B) Magnified images from (A). 5-HTR6s are not evenly distributed along the length of cilia and can be enriched at areas with low ADCY3 labeling (arrow).(C) Left panel: cilia (magenta) co-labeled with serotonergic axons (green). 20-µm maximum intensity projection (MIP). Middle panel: cilia in the left panel color coded by the shortest distance to a serotonergic axon. Right panel: cilia from left panel color coded with the shortest distance to a serotonergic axon-associated synaptophysin punctum.(D) Floxed synaptophysin-EGFP driven by Tph2-Cre showed ADCY3-labeled cilia (magenta in the merged panel) in contact with serotonergic presynaptic terminals (amplified by anti-GFP and Alexa 488, green in the merged panel).(E) 5-HTR6 (green in the merged panel) are enriched on the cilia at the axonal contact sites (SERT, cyan in the merged panel). Two cilia are in contact with a single serotonergic axonal varicosity. ADCY3 (magenta in the merged panel) can extend beyond the contact site that has few 5-HTR6 (arrow).(A), (B), and (E) are deconvolved confocal images with photon counting detection (Leica). (C) and (D) are Airyscan images (Zeiss). Data from 3- to 4-month-old male C57BL/6J mice.

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SERT Antibody

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Synaptic Systems GmbH's SERT Antibody is a Guinea Pig Polyclonal antibody. This antibody has been shown to work in applications such as: Immunohistochemistry, and Immunohistochemistry - fixed. The SERT Antibody was generated using SLC6A4 as the antigen and it reacts with Mouse, and Rat.

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Polyclonal Guinea pig antiserum

Acta neuropathologica communications

Cell

SERT Antibody from Synaptic Systems GmbH

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Synaptic Systems GmbH

Citations (3)

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SERT Antibody from Synaptic Systems GmbH

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