Purified anti-eIF3I (p36) 646701 from BioLegend

Product Specs
ItemPurified anti-eIF3I (p36)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number646701
NCBI Gene AliasesEIF3S2, PRO2242, TRIP-1, TRIP1, eIF3-beta, eIF3-p36
Conjugate/TagUnconjugated
ReactivityHuman
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
IsotypeIgG2a, Kappa
ApplicationsWestern Blot (WB)
Quantity25 µg
Clone17C11
HostMouse
Antibody TypePrimary
NCBI Full Gene Nameeukaryotic translation initiation factor 3 subunit I
Concentration0.50 mg/mL
ClonalityMonoclonal
TargeteIF3I

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Efficient CRISPR-Cas9-mediated mutagenesis in primary human B cells for identifying plasma cell regulatorsMolecular Therapy - Nucleic AcidsDecember 13, 2022Tuan Anh Le, Van Trung Chu, Andreia C. Lino, Eva Schrezenmeier, Christopher Kressler, Dania Hamo, Klaus Rajewsky, Thomas Dörner, Van Duc DangIRF4-PerCP/Cy5.5 (Clone IRF4.3E4, Biolegend, Cat. #646415, RRID: AB_2728481), BLIMP1/PRDM1-APC (Clone 646702, R&D Systems, Cat. #IC36081A, RRID: AB_11128645), XBP-1S-BV421 (Clone Q3-695, BD Biosciences, Cat. #563382,

(2) SARS-CoV-2 Omicron boosting induces de novo B cell response in humansbioRxivSeptember 26, 2022Wafaa B. Alsoussi et al.Nuclear Permeabilization/Wash buffer, stained with Ki-67-BV711 (Ki-67, BioLegend, 1:200), Blimp1-PE (646702, R&D, 1:100), FoxP3-Spark 685 (206D, BioLegend, 1:200), and Bcl6-R718 (K112-91, BD Horizon, 1:200) for

(3) Rad52 mediates class-switch DNA recombination to IgDNature communicationsFebruary 21, 2022Xu Y, Zhou H, Post G, Zan H, Casali P.647-anti-human IgD mAb (clone IA6-3, 348227, BioLegend) or Alexa Fluor® 488-anti-human BLIMP1 mAb (clone 646702, IC36081G, R&D Systems). FACS analysis was performed on single cell suspensions. In all flow cytometry

(4) SARS-CoV-2 infection induces long-lived bone marrow plasma cells in humansNatureJuly 1, 2021Turner JS, Kim W, Kalaidina E, Goss CW, Rauseo AM, Schmitz AJ, Hansen L, Haile A, Klebert MK, Pusic I, et al.A/Michigan/45/2015, DyLight 488- and Alexa 647-conjugated S, Ki-67-BV711 (Ki-67, 1:200, BioLegend), and Blimp1-A700 (646702, 1:50, R&D), washed twice with perm/wash buffer, and resuspended in P2. For memory B cell staining, PBMC

(5) Flow Cytometric Methods for the Detection of Intracellular Signaling Proteins and Transcription Factors Reveal Heterogeneity in Differentiating Human B Cell SubsetsCellsDecember 8, 2020Casper Marsman, Tineke Jorritsma, Anja ten Brinke, S. Marieke van HamFactors PAX5 PE 1H9 Biolegend 1:10.000 c-MYC AF647 D84C12 CST 1:150 13871S BCL6 PE-Cy7 7D1 Biolegend 1:400 358,512 BLIMP1 AF647 #646702 R&D 1:40 IC36081R-025 XBP-1s AF647 Q3-695 BD 1:40 562,821 AID AF647 EK2-5G9 BD 1:150 565,785 *

(6) Unlike for cellular mRNAs and other viral internal ribosome entry sites (IRESs), the eIF3 subunit e is not required for the translational activity of the HCV IRESThe Journal of biological chemistryFebruary 14, 2020Panthu B, Denolly S, Faivre-Moskalenko C, Ohlmann T, Cosset FL, Jalinot P.

(7) A threonyl-tRNA synthetase-mediated translation initiation machineryNature communicationsMarch 22, 2019Jeong SJ, Park S, Nguyen LT, Hwang J, Lee EY, Giong HK, Lee JS, Yoon I, Lee JH, Kim JH, et al.(A303–005A, 1:1000), eIF3G (A301–757A, 1:1000), and eIF3H (A301–754A, 1:1000) from Bethyl; eIF3I (#646701, 1:1000) and eIF3J (#638401, 1:1000) from Biolegend. Transfection was performed using Lipofectamine

(8) GSK3 Inhibition Drives Maturation of NK Cells and Enhances Their Antitumor ActivityCancer researchOctober 15, 2017Cichocki F, Valamehr B, Bjordahl R, Zhang B, Rezner B, Rogers P, Gaidarova S, Moreno S, Tuininga K, Dougherty P, et al.NKp80 (5D12), 2B4 (C1.7), TNF (MAb11), IFN-γ (clone B27) (all from Biolegend), NKG2C (134591), BLIMP1 (646702) (from R&D Systems), NKG2A (Z199) (from Beckman Coulter), ZEB2 (from Novus Biologicals), EOMES (WD1928),

(9) The Development of Plasmodium falciparum-Specific IL10 CD4 T Cells and Protection from Malaria in Children in an Area of High Malaria TransmissionFrontiers in immunologyJanuary 1, 2017Boyle MJ, Jagannathan P, Bowen K, McIntyre TI, Vance HM, Farrington LA, Schwartz A, Nankya F, Naluwu K, Wamala S, et al.(HIB19), and anti-T-Bet BV421 (4B10); from Milteny anti-γδVδ2-APC (123R3); from R&D anti-BLIMP-1 Alexa647 (646702); and from Invitrogen, Live/Dead aqua amine. Samples were acquired on a BD LSR2 flow cytometer with

(10) The spliceosome is a therapeutic vulnerability in MYC-driven cancerNatureSeptember 17, 2015Hsu TY, Simon LM, Neill NJ, Marcotte R, Sayad A, Bland CS, Echeverria GV, Sun T, Kurley SJ, Tyagi S, et al.(Cell Signaling, 9664), RPS8 (Assay Biotechnology, R12-3466), EIF2S1 (Abgent, AP13469s), eIF3I (p36) (Biolegend, 646701), and c-Myc (D84C12) (Cell Signaling, 5605). Vinculin (Sigma, V9131) and ran (BD Biosciences, 610340)

(11) eIF3 targets cell-proliferation messenger RNAs for translational activation or repressionNatureJune 4, 2015Lee AS, Kranzusch PJ, Cate JH.anti-eIF3g antibody (Bethyl A301-757A); anti-eIF3h antibody (Bethyl A301-754A); anti-eIF3i (Biolegend 646701); anti-eIF3k (Novus NB100-93304); anti-eIF3l antibody (Genetex GTX120119); anti-eIF3m (Novus NBP1-56654);

(12) Organic anion and cation transport in vitro by dog choroid plexus: effects of neuroleptics and tricyclic antidepressantsActa pharmacologica et toxicologicaFebruary 1, 1979E H Bárány

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Purified anti-eIF3I (p36) from BioLegend

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Purified anti-eIF3I (p36) from BioLegend

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