Fig 1: Detecting IFN production by fluorescence microscopyTHF IFN-GFP cells were mock infected, infected with UV-inactivated SeV (SeV-UV), UV-inactivated HCMV (HCMV-UV) or live SeV. Cells were fixed 16 h later and stained with ISG15 (red) to show ISG upregulation (A), pan-SeV antibody (red) to show SeV infection (B), GFP antibody (green) to boost GFP fluorescence and Hoechst (blue) to show nuclei (A and B). Fluorescence images were taken using a Zeiss Axio Vert microscope. Scale bars of 500 μm (A) and 125 μm (B) are included in the lower right corner. Figure re-printed with permission from the authors (Hare et al., 2020).