Fig 1: Immunofluorescence staining of proteins in thyroid cells using confocal microscopy before differentiation protocol. (A) TSHR (membrane) and (B) TTF1 (nuclear), Thyroid cells stained in green for all proteins and counterstained in blue for nuclei. The cytoskeleton was stained with phalloidin red to observe cell morphology. Objective 40 × original magnification, scale bars represent 50 µm. TSHR = thyroid stimulating hormone receptor, TTF1 = thyroid transcription factor 1.