Purified anti-Nuclear Pore Complex Proteins 902901 from BioLegend

Product Specs
ItemPurified anti-Nuclear Pore Complex Proteins
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number902901
Quantity100 µL
ApplicationsImmunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC)-P, Immunohistochemistry (IHC)-F, Immunoprecipitation (IP), IElectron Microscopy
Concentration1.00 mg/mL
Conjugate/TagUnconjugated
FormatPhosphate-buffered solution + 0.03% Thimerosal
ReactivityHuman, Mouse, Rat
IsotypeIgG1
CloneMAb414
HostMouse
ImmunogenThe antibody was raised using a nuclear pore complex mixture
ClonalityMonoclonal
Brand/TrademarkMMS-120P-100
TargetNuclear Pore Complex Proteins

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Website: biolegend.com

(1) Nuclear growth and import can be uncoupledbioRxivApril 25, 2023Pan Chen, Sampada Mishra, Daniel L. Levyand sealed with nail polish. The main primary antibody used was mouse mAb414 at 1:1000 (BioLegend, #902901), which recognizes NPC FG-repeats. Secondary antibodies included 1:1000 dilutions of Alexa Fluor 488–

(2) Differentiation-dependent changes in lamin B1 dynamics and lamin B receptor localizationMolecular Biology of the CellFebruary 1, 2023Chase C. Wesley, Daniel L. Levysc-376248), mouse anti–nuclear pore complex used at 1:1000 for immunofluorescence (mAb414; Biolegend; 902901), mouse anti-histone H3K9me3 used at 1:100 for immunofluorescence (Active Motif; 39285), goat anti-rabbit IgG H&L Alexa Fluor 405

(3) Nucleoporins facilitate ORC loading onto chromatinCell ReportsNovember 8, 2022Logan Richards, Christopher L. Lord, Mary Lauren Benton, John A. Capra, Jared T. Nordman600-401-914; RRID:AB_828383 Mouse Anti-Nuclear Pore Complex Proteins Antibody (mab414),BioLegend,Cat#: 902901; RRID:AB_2565026 Mouse Anti-H2B Antibody,Abcam,Cat#: ab52484; RRID:AB_1139809 Rabbit IgG,Sigma-Aldrich,Cat#:

(4) Nuclear F-actin and Lamin A antagonistically modulate nuclear shapeJournal of cell scienceJuly 1, 2022Mishra S, Levy DL.antibodies were used: anti-His-6× (1:500, Invitrogen, MA1-21315), anti-mouse mAb414 (1:1000, BioLegend, 902901) and anti-rabbit Nup53 (1:500, obtained from Richard Wozniak, University of Alberta, Edmonton, Canada;

(5) NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubilityNature communicationsJune 13, 2022Gleixner AM, Verdone BM, Otte CG, Anderson EN, Ramesh N, Shapiro OR, Gale JR, Mauna JC, Mann JR, Copley KE, et al.without DAPI stain prior to microscopy imaging. Primary antibodies: MAb414 (1:1000, Biolegend, Cat. No. 902901), NUP62 (1:400, Abcam, Cat. No. ab188413 or 1:500, Millipore, MABE1043), MAP2 (1:1000, Synaptic Systems,

(6) Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signalingThe Journal of clinical investigationApril 1, 2022Liu Z, Yan M, Lei W, Jiang R, Dai W, Chen J, Wang C, Li L, Wu M, Nian X, et al.sc-338; Santa Cruz Biotechnology); rabbit anti-GFAP (catalog AP0123; Ascend); mouse anti-mAb414 (catalog 902901; BioLegend); goat anti-Olig2 (catalog AF2418; R&D Systems); mouse anti-NeuN (catalog MAB377; MilliporeSigma);

(7) Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formationThe Journal of cell biologyFebruary 7, 2022Golchoubian B, Brunner A, Bragulat-Teixidor H, Neuner A, Akarlar BA, Ozlu N, Schlaitz AL.1/500 ELYS,"Sigma-Aldrich, HPA031658",Rabbit,"IF: 1/200; WB: 1/1,000" FG Nups (Mab414),"BioLegend, 902901",Mouse,"IF: 1/2,000" RanBP2,"Pichler et al., 2002",Goat,"IF: 1/4,000" Lamin A/C,"Santa Cruz, sc-6215",Goat,IF:

(8) O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transportThe Journal of cell biologyJuly 5, 2021Yoo TY, Mitchison TJ.#ab53775; Abcam), anti-RanGAP1 (1F3A4, 1:100, #67146-1-Ig; Proteintech), anti-FG-NUPs (MAb414, 1:1,000, #902901; BioLegend), anti-RanGTP (AR12, 1:200, gift from Ian Macara), anti-importin-β1 (3E9, 1:1,000, #MA3-070;

(9) OASIS/CREB3L1 is a factor that responds to nuclear envelope stressCell Death DiscoveryJune 29, 2021Yasunao Kamikawa, Atsushi Saito, Koji Matsuhisa, Masayuki Kaneko, Rie Asada, Yasunori Horikoshi, Satoshi Tashiro, Kazunori Imaizumi(1:250; M192-3 and #562 MBL Nagoya, Japan), anti‐Lamin B1 (1:1000; Abcam), mAB414 (1:100; Biolegend, #902901, San Diego, CA, USA), anti‐GFP (1:250; #598, MBL), anti-Nesprin-2 (1:100; MABC86, Millipore), anti-LAP2β

(10) T-cell receptor (TCR) signaling promotes the assembly of RanBP2/RanGAP1-SUMO1/Ubc9 nuclear pore subcomplex via PKC-θ-mediated phosphorylation of RanGAP1eLifeJune 10, 2021He Y, Yang Z, Zhao CS, Xiao Z, Gong Y, Li YY, Chen Y, Du Y, Feng D, Altman A, et al.#: ab129160, RRID:AB_11142853",WB (1:2000) Antibody,Mouse monoclonal anti- Mab414,BioLegend,"Cat #: 902901, RRID:AB_2565026",WB (1:1000) IF (1:200) Antibody,Rat monoclonal anti-mouse CD3,BioLegend,"Cat #: 100202,

(11) Drosophila female germline stem cells undergo mitosis without nuclear breakdownCurrent biology : CBApril 12, 2021Duan T, Cupp R, Geyer PK.

(12) Molecular and Phenotypic Characterization following RNAi Mediated Knockdown in DrosophilaBIO-PROTOCOLFebruary 20, 2021Saurabh Jayesh Kumar Mehta, Pradyumna A Joshi, Ram Kumar Mishra621650500501730) 46. Fluoroshield with DAPI (Sigma-Aldrich, catalog number: F6057) 47. mAb414 (Biolegend 902901) - mAb414 recognizes FG-repeat rich four distinct nucleoporins of nuclear pores 48. Anti-dElys (Mehta

(13) DNA content contributes to nuclear size control in Xenopus laevisMolecular biology of the cellNovember 15, 2020Heijo H, Shimogama S, Nakano S, Miyata A, Iwao Y, Hara Y.above-mentioned coverslips with sedimented nuclei were stained using α-NPC antibody (mAb414; BioLegend; 902901). Imaging of DiOC6(3) signals and NPCs on the nuclei was performed using the above-mentioned wide-field

(14) The Perinuclear ER Scales Nuclear Size Independently of Cell Size in Early EmbryosDevelopmental cellAugust 10, 2020Mukherjee RN, Sallé J, Dmitrieff S, Nelson KM, Oakey J, Minc N, Levy DL.antibodies: rabbit anti-KTN1 at 1:50 (Sigma #K1644) and mouse anti-NPC mAb414 at 1:250 (BioLegend # 902901) in WMBS. Following 5× 1 hour washes in TBST at room temperature, blastomeres were blocked overnight

(15) Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase SignalingMolecular cellJuly 16, 2020Wigington CP, Roy J, Damle NP, Yadav VK, Blikstad C, Resch E, Wong CJ, Mackay DR, Wang JT, Krystkowiak I, et al.

(16) Nucleoplasmin is a limiting component in the scaling of nuclear size with cytoplasmic volumeThe Journal of cell biologyDecember 2, 2019Chen P, Tomschik M, Nelson KM, Oakey J, Gatlin JC, Levy DL.Vector Laboratories) onto glass slides and sealed with nail polish. Primary antibodies included mAb414 (902901, mouse, 1:1,000; BioLegend) that recognizes NPC FG repeats, histone H2B antibody (07-371, rabbit, 1:100;

(17) Nuclear formation induced by DNA-conjugated beads in living fertilised mouse eggScientific ReportsDecember 1, 2019Yuka Suzuki, Şükriye Bilir, Yu Hatano, Tatsuhito Fukuda, Daisuke Mashiko, Shouhei Kobayashi, Yasushi Hiraoka, Tokuko Haraguchi, Kazuo Yamagata(Monoclonal Antibody Laboratory Inc.; MABI0400); FG-Nups of the NPC (mAb414, BioLegend, San Diego, CA, USA; 902901); ELYS (Abcam, Cambridge, UK; ab14431); Ran (BD Biosciences, Franklin Lakes, NJ, USA; BD610341); and

(18) Chromatin targeting of nuclear pore proteins induces chromatin decondensationThe Journal of cell biologySeptember 2, 2019Kuhn TM, Pascual-Garcia P, Gozalo A, Little SC, Capelson M.Rockland Inc. at 1:100; Myc 9E10/sc-40X from Santa Cruz Biotechnology at 1:100; mAb414 (NPC marker) #902901 from Biolegend at 1:20; H3 #39763 from Active Motif at 1:100; HOAP from the Yikang S. Rong Lab (Sun

(19) Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the poreThe Journal of cell biologySeptember 2, 2019Ben-Yishay R, Mor A, Shraga A, Ashkenazy-Titelman A, Kinor N, Schwed-Gross A, Jacob A, Kozer N, Kumar P, Garini Y, et al.

(20) "Pathogenic Variants in NUP214 Cause ""Plugged"" Nuclear Pore Channels and Acute Febrile Encephalopathy"American journal of human geneticsJuly 3, 2019Fichtman B, Harel T, Biran N, Zagairy F, Applegate CD, Salzberg Y, Gilboa T, Salah S, Shaag A, Simanovsky N, et al.

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Purified anti-Nuclear Pore Complex Proteins from BioLegend

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Purified anti-Nuclear Pore Complex Proteins from BioLegend

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