GOAT ANTI HUMAN IgM

Sandwich ELISA analysis of IgM binding using Mouse anti Human IgM antibody, clone M15/8 as a capture reagent and biotinylated Goat anti Human IgM antibody (AHP1764) as a detection regent with purified human IgM as antigen for the generation of the standard curve. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Serum samples at 1:10k (green) and 1:100k (blue), similarly plasma samples 1:10k (orange) and 1:100k (red) dilutions are shown.

Sandwich ELISA analysis of IgM binding using Mouse anti Human IgM antibody, clone M15/8 (MCA1662) as a capture reagent and biotinylated Goat anti Human IgM antibody (AHP1764) as a detection regent with purified human IgM (5275-5504) as antigen for the generation of the standard curve. Subsequent detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples at 1:10k (green) and 1:100k (blue), similarly plasma samples 1:10k (orange) and 1:100k (red) dilutions are shown.