Fig 1: GRIA2 interacted with ENPP3 and regulated the proliferation and migration of VSMCs. (A,B) siENPP3 and ENPP3 plasmids and siNC and shRNA NC plasmids were transfected into GRIA2 lentivirus-transfected VSMCs. The EdU assay illustrated the proliferation of VSMCs (scale bar, 50μm; n=3). (C,D) Transwell assays illustrated the migration of VSMCs (scale bar, 100μm; n=3). Images are representative, and the bar graph shows the average data of four independent experiments (n=3). Images of EdU and Transwell migration assays were taken at 200× magnification. Data are presented as the mean±SEM. **Statistically significant difference (p<0.01) and ***statistically significant difference (p<0.001). VSMCs: vascular smooth muscle cells.
Fig 2: Integrated analysis of GEO datasets identified DEGs. (A) Venn diagrams of common DGEs between two combined datasets (GSE23314 and GSE53274). Each circle represents a dataset, and the overlap between the circles is the overlap between the datasets. (B) PPI network integration of the common DGEs. Each node is a gene, and the connections between nodes represent the interaction of these biological molecules. ENPP3 (black arrow).
Fig 3: GRIA2 interacted with ENPP3 in restenosis. (A) Immunohistochemical analysis and quantitative mRNA analysis showed the expression of ENPP3 in restenotic plaque tissues, atherosclerotic plaque tissues and healthy control artery tissues (n=3). Optical density (OD) values are the statistical calculations in the intima. Images are representative, and the bar graph shows the average data of three independent experiments (n=3). Staining was performed at 200×magnification (scale bar, 50μm). (B) The expression of ENPP3 in VSMCs after upregulating GRIA2 expression. Blots are representative, and the bar graph shows the average data (n=3). *Statistically significant difference (p<0.05), **statistically significant difference (p<0.01), and ***statistically significant difference (p<0.001). AS: atherosclerotic; RS: restenotic; CON: control; VSMCs: vascular smooth muscle cells.
Supplier Page from Abcam for Anti-ENPP3/B10 antibody