Fig 1: NetOGlyc analysis of the potential O-glycosylation sites on Ser/Thr residues in the selected seminal plasma glycoproteins. Residues with NetOGlyc 4.0 scores >0.5 indicate predicted O-linked glycans. Glycoproteins analyzed: (A)—SEMG1; (B)—SEMG2; (C)—PIP; (D)—FN; (E)—MUC6; (F)—LTF.
Fig 2: Protein and immunoreactivity patterns of the lectin affinity chromatography isolates. Lane 1: lectin probed whole seminal plasma; 2: silver stained whole seminal plasma; 3: 0.2 M GalNAc released glycoproteins, silver stained; 4: Gly-HCl buffer released glycoproteins, silver stained; Lanes 5–9: immunoreactivity of the isolates; 5: a-FN; 6: a-SEMG1; 7: a-SEMG2; 8: a-PIP; 9: a-LTF. (A)—VVL-agarose column, (B)—MPL-agarose column.
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