Fig 1: Biocompatibility test of T-ECH.a, b Representative immunofluorescence images of the sciatic nerve 14 days after implantation with T-ECH, sham group, and stainless steel (S-100 in red; NFM in yellow; Iba1 in green; DAPI in blue). Scale bar = 100 µm. c–e Normalized fluorescence intensity of T-ECH, stainless steel, and sham group respectively for (c) S-100, (d) NFM, and (e) Iba1. Data are plotted as mean values and the error bar represents the standard deviation. One-way ANOVA and Tukey’s multiple comparison tests were employed for statistical analysis; *P < 0.05 ***P < 0.001 with a confidence level of 95%. (n = 5 devices examined with individual mice for S100 and NFM of sham. n = 7 for S100 and NFM of T-ECH and stainless steel, n = 7 for Iba1 of all groups. For comparison of S-100 intensity, P = 0.03 for both between T-ECH and stainless steel and between Sham and stainless steel. For comparison of Iba1 intensity, P = 0.0004 for both between T-ECH and stainless steel and between Sham and stainless steel).
Fig 2: a) Fluorescence images of live (green)/dead (red) 3T3 fibroblasts cells cultured with a petri dish (CT), the cold-drawn PCL fiber, the biodegradable fiber electrode, and DMSO for 7 days. b) Cell viability on the cold-drawn PCL fiber and biodegradable fiber electrode measured using the CCK-8 assay for 7 days. Data are presented as mean ± SD (n = 3). c) The IHC staining images of mice sciatic nerve for NFM (neurofilament) and S100 (Schwann cell) (blue: DAPI, red: S100, green: NFM) after 6-day implantation of the control, bare PCL fiber, and the biodegradable fiber electrode. DAPI was used as nucleic acid marker. d) Quantification of the percentage of cells that are S-100 positive in sciatic nerve from mice in control, bare PCL fiber and fiber electrode group. Data are presented as mean ± SD (n = 3), and statistical analysis proceeded with one-way ANOVA (P > 0.05; ns: no significant difference). e) Photographs of the biodegradable fiber electrode at various states of dissolution in PBS (pH 7.4, 55 °C). f) Weight loss of the biodegradable fiber electrode in PBS (pH 7.4, 37°C (yellow), and 55 °C (red)). Data are presented as mean ± SD (n = 3). g) Change in the electrical resistance of the biodegradable fiber electrode with and without the PPC buffer coating layer in PBS (pH 7.4, 37 °C) over time. Data are presented as mean ± SD (n = 3). h) SEM images of the biodegradable fiber electrode in PBS (pH 7.4, 37 °C) at 0 and 28 days, showing the change of the surface morphologies of the biodegradable fiber electrode.
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