Fig 2: ABI3BP is poorly expressed in GBC tissues and cells. a, microarray data analysis of GSE74048 dataset. The abscissa indicates the sample type and the ordinate indicates the differential genes. Histogram on the upper right represents color gradation and rectangle refers to the expression level. b, the correlation analysis between expression of MALAT1 and ABI3BP in GBC tissues; c, the relative expression of ABI3BP in normal gallbladder tissues (n = 16) and GBC tissues (n = 48) detected by immunohistochemistry (400 ×). d, the relative expression of ABI3BP in the GBC cells (GBC-SD, SGC-996, NOZ and OCUG1) and HGBECs. * p < 0.05 vs. the normal gallbladder tissue or HGBEC cell line. The data was measurement data and expressed as mean value ± standard deviation. Comparison was analyzed by independent sample t-test between two groups and by one-way analysis of variance among multiple groups, followed by Turkey’s post-hoc test. The experiment was repeated 3 times. ABI3BP, ABI family member 3 binding protein; GBC, gallbladder cancer; HGBECs, human gallbladder epithelial cells

Fig 3: ABI3BP over-expression restrains the proliferation and metastasis of GBC cells while contributes to senescence. a and b, the proliferation of GBC-SD cells detected by EdU assay (200 ×). c and d, the protein expression of Ki67 and PCNA relative to ß-actin in GBC-SD cells determined by Western blot analysis. e, the migration of GBC-SD cells detected by scratch test. f, the invasion of GBC-SD cells detected by Transwell assay (200 ×). g, the senescence of GBC-SD cells detected by SA-ß-gal staining (400 ×). * p < 0.05 vs. the GBC cells manipulated with vector-NC. The data was measurement data and expressed as mean value ± standard deviation. Comparison was analyzed by independent sample t-test. The experiment was repeated 3 times. ABI3BP, ABI family member 3 binding protein; GBC, gallbladder cancer; EdU, 5-ethynyl-2'-deoxyuridine; PCNA, proliferating cell nuclear antigen; SA-ß-gal, senescence-associated ß-galactosidase; NC, negative control

Fig 4: Suppression of proliferation, invasion, migration and tumorigenic potential induced by MALAT1 silencing could be rescued by ABI3BP silencing. a, the proliferation determined by EdU assay (200 ×). b, the cell migration detected by scratch test. c, the cell invasion detected by Transwell assay (200 ×). d, representative images of resected tumors from nude mice and tumor weights. e, the relative expression of ABI3BP determined by immunohistochemistry (400 ×). * p < 0.05 vs. cells or nude mice treated with sh-MALAT1. The data was measurement data and expressed as mean value ± standard deviation. Comparison was analyzed by independent sample t-test between two groups. The experiment was repeated 3 times

Fig 5: MALAT1 down-regulates the expression of ABI3BP through EZH2 recruitment in the promoter region of ABI3BP. a, the subcellular localization of MALAT1 predicted on lncATLAS website. b, Blast comparison between sequences of MALAT1 and ABI3BP promoter. c, the subcellular localization of MALAT1 detected by FISH assay (400 ×). d, the enrichment of EZH2 by MALAT1 detected by RIP assay. e, the relative expression of EZH2 relative to β-actin detected by RNA pull down. f, EZH2 enrichment in the ABI3BP promoter region detected by ChIP assay. g, the relative expression of ABI3BP determined by RT-qPCR. * p < 0.05 vs. the cells treated with sh-NC, Bio-probe-NC or DMSO. The data was measurement data and expressed as mean value ± standard deviation. Comparison was analyzed by independent sample t-test between two groups and by one-way analysis of variance among multiple groups, followed by Turkey’s post-hoc test. The experiment was repeated 3 times. MALAT1, metastasis associated lung adenocarcinoma transcript 1; ABI3BP, ABI family member 3 binding protein; EZH2, enhancer of zeste homolog 2; FISH, fluorescence in situ hybridization; RIP, RNA immunoprecipitation; ChIP, chromatin immunoprecipitation; RT-qPCR, reverse transcription quantitative polymerase chain reaction; NC, negative control; DMSO, dimethyl sulfoxide