Fig 1: miR-382-5p overexpression retarded cell proliferation, metastasis while facilitated apoptosis in NSCLC cells by targeting SPIN1. (A–H) The H1299 and A549 cells were transfected with miR-NC, miR-382-5p, miR-382-5p + pcDNA, or miR-382-5p + pcDNA-SPIN1. (A) The expression of SPIN1 was detected by qRT-PCR. (B and C) The protein level of SPIN1 was measured via Western blot assay. (D and E) The cell viability was estimated by MTT assay. (F) The apoptotic rate was assessed through flow cytometry. (G and H) The migrated and invaded capacities were evaluated via Transwell assay. *P<0.05.
Fig 2: SNHG14 silencing impeded SPIN1 expression by sponging miR-382-5p in NSCLC cells. (A and B) The NSCLC cells were transfected with si-NC, si-SNHG14, si-SNHG14 + inhibitor-NC, or si-SNHG14 + miR-382-5p inhibitor. (A) The level of SPIN1 mRNA was examined by qRT-PCR. (B) The level of SPIN1 protein was examined via Western blot assay. *P<0.05.
Fig 3: SPIN1 was a direct target of miR-382-5p in NSCLC cells. (A) The complementary sequences between SPIN1 3'UTR and miR-382-5p were presented, as well as the mutant sequences of SPIN1 3'UTR. (B and C) The luciferase activity of SPIN1 3'UTR-WT or SPIN1 3'UTR-MUT reporter in NSCLC cells transfected with miR-NC or miR-382-5p was evaluated by dual-luciferase reporter assay. (D and E) The H1299 and A549 cells were transfected with miR-382-5p or miR-NC. (D) The expression of SPIN1 was tested via qRT-PCR. (E) The level of SPIN1 protein was measured by Western blot assay. (F–I) The levels of SPIN1 mRNA and protein in NSCLC tissues and cells were monitored via qRT-PCR and Western blot assay, respectively. (J) The correlation between SPIN1 and miR-382-5p was processed by Pearson test. *P<0.05.
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