Fig 1: IRF5 promoted the binding of transcription factors SP1, PU.1 to glycolytic gene promoters. (A) The binding of SP1 to glycolytic gene promoters HK1, TPI1, PGAM1, PFKFB3 and positive controls IL-6, IFN-a after siRNA-IRF5 intrathecally in CCI mice by ChIP; (B) the binding of PU.1 to glycolytic gene promoters and positive controls after siRNA-IRF5 intrathecally in CCI mice by ChIP; (C) the binding of SP1 to glycolytic gene promoters and positive controls after siRNA-IRF5 administration in cultured primary microglia by ChIP; (D) the binding of PU.1 to glycolytic gene promoters and positive controls after siRNA-IRF5 administration in cultured primary microglia by ChIP.
Fig 2: CCI-induced glycolysis enhancement was suppressed by Bafetinib or siRNA-lyn intrathecally. (A) Representative images of protein expressions of key enzymes in glycolysis (HK1, TPI1, PGAM1 and PFKFB3) in CCI mice; (B) statistical analysis of protein expressions of key enzymes in glycolysis in CCI mice; (C) mRNA levels of key enzymes in glycolysis in CCI mice; (D) lactate level in spinal dorsal horn of CCI mice by microdialysis; (E) representative images of protein expressions of key enzymes in glycolysis after Bafetinib and siRNA-lyn intrathecally in CCI mice; (F) statistical analysis of protein expressions of key enzymes in glycolysis after Bafetinib and siRNA-lyn intrathecally in CCI mice; (G) mRNA levels of key enzymes in glycolysis after Bafetinib and siRNA-lyn intrathecally in CCI mice; (H) lactate level after Bafetinib and siRNA-lyn intrathecally in spinal dorsal horn of CCI mice by microdialysis; (I) representative images of protein expressions of key enzymes in glycolysis after Bafetinib and siRNA-lyn administration in cultured primary microglia; (J) statistical analysis of protein expressions of key enzymes in glycolysis after Bafetinib and siRNA-lyn administration in cultured primary microglia; (K) mRNA levels of key enzymes in glycolysis after Bafetinib and siRNA-lyn administration in cultured primary microglia; (L) lactate level in cultured primary microglia after Bafetinib and siRNA-lyn administration.
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