Fig 1: OA regulated TBX20/PROK2 signaling pathway. Western blotting analysis of TBX20 or PROK2 in HUVECs stimulated with DMSO, 2.5 µM OA (30, 60 min) and VEGFA165 (100 ng/ml), ß-actin expression an internal control of protein loading was performed, with VEGFA165 as a positive control (A). Quantitative data included TBX20 (B) and PROK2 (C). TBX20 (D) and PROK2 (E) mRNA expression in HUVECs treated with DMSO or 2.5 µM OA for 30, 60 min were analyzed using qRT-PCR. Statistical analysis of TBX20 (F) and PROK2 (G) mRNA of HUVECs transfected with non-specific siRNA or ROCK-II siRNA and stimulated with DMSO or 2.5 µM OA for 30, 60 min were presented. Data were represented as mean ± SD (n = 3). *p < 0.05, **p < 0.01 vs. control siRNA; & p < 0.05 vs. ROCK-II siRNA; # p < 0.05 vs. control siRNA + OA 60 min.
Supplier Page from Abcam for Anti-Tbx20 antibody