Fig 1: Convallatoxin increases osteogenic differentiation of os cells through suppressing pthr1 expression and Wnt/ß-catenin pathway. mg63 and u2os cells treated with convallatoxin were transfected with ov-PTHR1 or sh-dkk1. (a, b) western blot assay for determination of collagen 1, osteopontin, rankl, runx2, osteocalcin expressions. *** p < 0.001 vs control, ## p < 0.01, ### p < 0.001 vs convallatoxin + ov-nc, d p < 0.05, dd p < 0.01, ddd p < 0.001 vs convallatoxin + sh-nc.
Fig 2: Overexpression of pthr1 activates Wnt/ß-catenin pathway. (a, b) mg63 and u2os cells were transfected with ov-pthr1 or ov-nc. rt-qpcr for determination of pthr1 level. *** p < 0.001 vs ov-nc (c, d) mg63 and u2os cells treated with convallatoxin were transfected with ov-pthr1 or ov-nc. western blot assay for determination of pthr1, dkk1 and ß-catenin expressions. *** p < 0.001 vs control, ## p < 0.01, ### p < 0.001 vs convallatoxin + ov-nc.
Fig 3: Convallatoxin suppresses pthr1 expression and Wnt/ß-catenin pathway. mg63 and u2os cells were treated with 0, 12.5, 25 and 50 nm convallatoxin for 24 h. (a, b) western blot assay for determination of pthr1, dkk1 and ß-catenin expressions. * p < 0.05, ** p < 0.01, *** p < 0.001 vs 0 nm convallatoxin.
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