Fig 1: MTH1 expression in liver tissues. (A) Immunostaining of MTH1. The black arrows indicate inflammatory cells, and the white arrows indicate proliferated biliary tracts. (B) Relative mRNA expression of MTH1 (***p < 0.001). (C) Western blot analysis of MTH1. (D) Kaplan–Meier analysis indicated statistically significantly shorter survival of patients in the MTH1 high expression group than the low expression group. P refers to paracancerous liver tissues and H refers to HCC tissues (*p < 0.05, **p < 0.01, ***p < 0.001).
Fig 2: Identification of NUDT1 as an oncogene in GC. a–e EdU assays (a, b), CCK8 assays (c) and colony formation assays (d, e) demonstrate that up-regulation of NUDT1 promotes cell proliferation of GC cells. f–i Transwell assays (f, g) and wound healing assays (h, i) show that overexpression of NUDT1 enhances cell migration of GC cells strongly. PCDNA NUDT1 refers to overexpression plasmid, and PCDNA NC refers to control plasmid. **P < 0.01
Fig 3: The effects of miR-485-5p/NUDT1 axis on normal gastric cells. a Quantitative RT-PCR analysis of miR-485-5p levels in GES-1 cells transfected with miR-485-5p mimics or inhibitors. b Relative NUDT1 mRNA levels after transfection. c The regulation of NUDT1 expression by miR-485-5p. d–f The up-regulation of miR-485-5p had no effect on the proliferation (d) and migration (e, f) of GES-1 cells, whereas the knockdown of miR-485-5p could promote malignant phenotype. *P < 0.05; **P < 0.01
Fig 4: The impacts of miR-485-5p/NUDT1 axis on eradicating 8-oxo-dG in GC cells. The 8-oxo-dG in the nucleotide pools was detected by immunofluorescence. When NUDT1 was up-regulated by plasmid or miR-485-5p inhibitors, the 8-oxo-dG in nucleus and cytoplasm decreased; while the level of 8-oxo-dG increased after the knockdown of NUDT1. PCDNA NUDT1 refers to overexpression plasmid, and PCDNA NC refers to control plasmid
Fig 5: MTH1 expression in hepatocellular carcinoma (HCC) in The Cancer Genome Atlas (TCGA) database. (A) Relative mRNA expression of MTH1. (B) Kaplan–Meier analysis of overall survival for the expression of MTH1. (C, D) MTH1 expression in different tumor node metastasis (TNM) stages and histologic grades. (E) Multivariate Cox analysis for the survival of HCC patients (*p < 0.05, **p < 0.01, ***p < 0.001).
Supplier Page from Abcam for Anti-MTH1 antibody [EPR15934]