Nitrotyrosine Antibody A-21285 from Thermo Fisher Scientific

Product Specs
ItemNitrotyrosine Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA-21285
Quantity500 µL
ApplicationsImmunocytochemistry, Immunohistochemistry, Western Blot
Conjugate/TagUnconjugated
FormatPBS with 5mM sodium azide; pH 7.2
ReactivityChemical
TargetNitrotyrosine
IsotypeIgG
HostRabbit
ImmunogenKeyhole limpet hemocyanin (KLH) containing nitrated tyrosines
ClonalityPolyclonal
Concentration1 mg/mL
Storage Temperature4° C
Antibody TypePrimary
Storage4° C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Unraveling the relationship among inflammatory responses, oxidative damage, and host susceptibility to Opisthorchis viverrini infection: A comparative analysis in animal modelsVeterinary WorldNovember 19, 2023Sirikachorn Tangkawattana, Watcharapol Suyapoh, Nathamon Taiki, Paramin Tookampee, Ravisara Chitchak, Theerayut Thongrin, Prasarn Tangkawattanawas assessed by incubating the sections overnight with polyclonal rabbit anti-nitrotyrosine antibody (A21285, 1:50; Thermo Fisher Scientific, USA), followed by staining using a DAKO ChemMate Envision Kit (Dako,

(2) Mitochondrial Dysfunction After Repeated Mild Blast Traumatic Brain Injury Is Attenuated by a Mild Mitochondrial Uncoupling ProdrugJournal of NeurotraumaNovember 1, 2023W. Brad Hubbard, Hemendra J. Vekaria, Gopal V. Velmurugan, Olivia J. Kalimon, Paresh Prajapati, Emily Brown, John G. Geisler, Patrick G. Sullivan96-well Bio-Dot microfiltration apparatus (Bio-Rad). Nitrotyrosine polyclonal antibody (1:2500; catalog #A-21285, Thermofisher) and anti-4 HNE antiserum (1:2500; catalog #HNE11-S, Alpha Diagnostic International, San

(3) The pro- and antineoplastic effects of deoxycholic acid in pancreatic adenocarcinoma cell modelsMolecular biology reportsJune 1, 2023Schwarcz S, Kovács P, Kovács T, Ujlaki G, Nyerges P, Uray K, Bai P, Mikó E.(NB300-605),1:1000 4-HNE,Abcam (ab46545),1:1000 ALDH1,Abcam (ab227948),1:1000 NITROTYROSINE,Thermo Fisher (A21285),1:1000 ACTIN,Sigma-Aldrich (A3854),"1:20,000" Antibodies used in Western blot analyses

(4) Attenuation of Tumor Burden in Response to Rucaparib in Lung Adenocarcinoma: The Contribution of Oxidative Stress, Apoptosis, and DNA DamageInternational journal of molecular sciencesJanuary 30, 2023Pérez-Peiró M, Valentí-Serra P, León-González B, Ampurdanés C, Duran X, Yélamos J, Barreiro E.antibody,1:2000,MD20A-G1a,"Academy Bio-Chemical Company, Houston, TX, USA)" Anti-3-nitrotyrosine antibody,1:1000,A-21285,"Thermo Fisher Scientific, Waltham, MA, USA" Protein carbonyl assay kit,1:5000,Ab178020,"Abcam, Cambridge,

(5) The Role of Acetyl Zingerone and Its Derivatives in Inhibiting UV-Induced, Incident, and Delayed Cyclobutane Pyrimidine DimersAntioxidants (Basel)January 26, 2023Jyoti Srivastava, Montana M. Young, Vipin Kumar Yadav, Pravin R. Phadatare, Thomas A. Meyer, Ratan K. Chaudhuri, Sanjay Premiat room temperature, cells were incubated with 1:1000 dilution of anti-nitrotyrosine antibody (Cat. A-21285, ThermoFisher Scientific) overnight at 4 °C. Detection was conducted using Alexa Fluor 488 Goat Anti-rabbit

(6) α-Syn overexpression, NRF2 suppression, and enhanced ferroptosis create a vicious cycle of neuronal loss in Parkinson's diseaseFree Radical Biology and MedicineNovember 1, 2022Anandhan Anandhan, Chen Chen, Nguyen Nguyen, Madhavan Madhavan, Dodson Dodson, D.D. Zhangab-46545) were from Abcam (Cambridge, UK). Anti-α-Syn (1:1000, AHB0261) and anti-nitrotyrosine (1:500, A-21285) were from Life Technologies (Carlsbad, CA). α-Syn (clone 42, 1:1000, 610787) was from BD Biosciences

(7) Investigating key factors underlying neurodegeneration linked to alphaâ€synuclein spreadNeuropathology and Applied NeurobiologyOctober 1, 2022Cindy C. C. Pang, Maja H. SÃ¸rensen, Krit Lee, Kelvin C. Luk, John Q. Trojanowski, Virginia M. Y. Lee, Wendy Noble, Raymond C. C. Chang8‐hydroxy‐2‐deoxyguanosine (Abcam #ab62623, 1:150) and anti‐nitrotyrosine antibody (Thermo scientific #A‐21285, 1:500) [47]. Western blot Western blot was performed following determination of sample protein concentration

(8) The role of Iron in lipid peroxidation and protein nitration during acetaminophen-induced liver injury in miceToxicology and Applied PharmacologyJune 1, 2022Olamide B. Adelusi, Anup Ramachandran, John J. Lemasters, Hartmut Jaeschkerabbit anti-4-hydroxynonenal (Abcam, ab46545) or rabbit anti-nitrotyrosine antibodies (Invitrogen, A-21285). Hydrogen peroxide (3%) was used to block endogenous peroxidase activity and an avidin-biotin conjugate

(9) Nutraceutical Strategy to Counteract Eye Neurodegeneration and Oxidative Stress in Drosophila melanogaster Fed with High-Sugar DietAntioxidants (Basel, Switzerland)July 27, 2021Catalani E, Fanelli G, Silvestri F, Cherubini A, Del Quondam S, Bongiorni S, Taddei AR, Ceci M, De Palma C, Perrotta C, et al.caspase 3 (#9664) was purchased from Cell Signaling Technology (Danvers, MA, USA); anti-nitrotyrosine (#A-21285) was obtained from Invitrogen-ThermoFisher Scientific (Monza, Italy); anti-Light-Chain 3 (LC3) (#ab128025)

(10) Curcumin Decreases Hippocampal Neurodegeneration and Nitro-Oxidative Damage to Plasma Proteins and Lipids Caused by Short-Term Exposure to OzoneMoleculesJuly 3, 2021María Luisa Mendoza-Magaña, Hugo Alejandro Espinoza-Gutiérrez, Sendar Daniel Nery-Flores, Abraham Alberto Ramírez-Mendoza, Cesar Ricardo Cortez-Álvarez, Robert de Mario Bonnet-Lemus, Mario Alberto Ramírez-Herreramembranes were washed with 0.1% PBS-Tween and incubated overnight with rabbit anti-3-NT (1:2000, Invitrogen A21285, Carlsbad, CA, USA) at 4 °C. After 5 washes, membranes were incubated with HRP labeled goat anti-rabbit

(11) Inducible Slc4a11 Knockout Triggers Corneal Edema Through Perturbation of Corneal Endothelial PumpInvestigative ophthalmology & visual scienceJune 1, 2021Ogando DG, Shyam R, Kim ET, Wang YC, Liu CY, Bonanno JA.(Thermo Fisher Scientific #33-9100), rabbit anti-Nitrotryrosine (1:200) (Thermo Fisher Scientific #A21285) were added in 2% BSA in PBSCM and incubated overnight at 4°C. Tissues were washed three times in PBSCM,

(12) Interplay of two transcription factors for recruitment of the chromatin remodeling complex modulates fungal nitrosative stress responseNature communicationsMay 6, 2021Jian Y, Liu Z, Wang H, Chen Y, Yin Y, Zhao Y, Ma Z.The membrane was then probed with Rabbit polyclonal anti-nitrotyrosine antibody at 1:1000 dilution (A-21285, Thermo Scientific) and washed five times with TBST. The membrane was further incubated with horseradish

(13) Long-term dietary nitrate supplementation does not reduce renal cyst growth in experimental autosomal dominant polycystic kidney diseasePloS oneJanuary 1, 2021Zhang JQJ, Saravanabavan S, Cheng KM, Raghubanshi A, Chandra AN, Munt A, Rayner B, Zhang Y, Chau K, Wong ATY, et al.anti-phospho-histone H2A.X (Ser139) (1:480, 9718; Cell Signaling Technology, Danvers, MA USA); and (v) anti-nitrotyrosine (1:500, A-21285; Invitrogen). Secondary biotinylated or HRP-conjugated antibodies (1:200 dilution) were applied for

(14) Hormetic-Like Effects of L-Homocysteine on Synaptic Structure, Function, and Aβ AggregationPharmaceuticals (Basel, Switzerland)February 2, 2020Montecinos-Oliva C, Arrázola MS, Jara C, Tapia-Rojas C, Inestrosa NC.[12]. 4. Materials and Methods 4.1. Antibodies The primary antibodies used were anti-3-nitrotyrosine (A21285, Invitrogen, Waltham, USA), goat anti-4HNE (H6275-02, US Biological, Life Sciences, Salem, USA), mouse

(15) Mice deficient in pyruvate dehydrogenase kinase 4 are protected against acetaminophen-induced hepatotoxicityToxicology and applied pharmacologyJanuary 15, 2020Duan L, Ramachandran A, Akakpo JY, Woolbright BL, Zhang Y, Jaeschke H.CA) and a rabbit polyclonal anti-nitrotyrosine antibody (Life Technologies, Grand Island, NY; Cat. # A-21285). Western Blotting Snap frozen tissue was homogenized in a CHAPs containing protein buffer and total

(16) Antioxidant Solution in Combination with Angiotensin-(1-7) Provides Myocardial Protection in Langendorff-Perfused Rat HeartsOxidative medicine and cellular longevityJanuary 1, 2020Andrä M, Russ M, Jauk S, Lamacie M, Lang I, Arnold R, Brcic I, Santos R, Wintersteiger R, Ortner A.antibodies against 4-hydroxynonenal (4-HNE) (ab46545, 1 : 200, Abcam, Cambridge, MA, USA) and nitrotyrosine (A21285, 1 : 200, Thermo Fisher Scientific, Waltham, MA, USA) was performed. 4-HNE was stained on DAKO Autostainer

(17) Investigation of antioxidative effects of a cardioprotective solution in heart tissueMolecular and cellular biochemistryNovember 1, 2019Russ M, Jauk S, Wintersteiger R, Andrä M, Brcic I, Ortner A.hematoxylin and eosin (HE), and immunohistochemically stained with antibodies against nitrotyrosine (A21285, 1:200, Thermo Fischer Scientific, Waltham, MA, USA) and 4-Hydroxynonenal (4-HNE) (ab46545, 1:200, AbCam,

(18) Inhibition of EZH2 methyltransferase decreases immunoediting of mesothelioma cells by autologous macrophages through a PD-1-dependent mechanismJCI insightSeptember 19, 2019Hamaidia M, Gazon H, Hoyos C, Hoffmann GB, Louis R, Duysinx B, Willems L.temperature with rat anti–mouse F4/80 (Becton Dickinson, clone T45-2342) and rabbit anti-nitrotyrosine (A-21285, Thermo Fisher Scientific) antibodies. Then, cells were stained with Alexa Fluor 488 anti–rat IgG and

(19) Apocynin and Tempol ameliorate dietary sodium-induced declines in cutaneous microvascular function in salt-resistant humansAmerican journal of physiology. Heart and circulatory physiologyJuly 1, 2019Ramick MG, Brian MS, Matthews EL, Patik JC, Seals DR, Lennon SL, Farquhar WB, Edwards DG.VE Cadherin (1:200; Abcam cat. no. ab7047, RRID:AB_2077943) and NT (1:100; Molecular Probes cat. no. A-21285, RRID:AB_221457), then incubated with Alexa Fluor secondary antibodies (1:500; Abcam anti-mouse IgG,

(20) TFEB-driven lysosomal biogenesis is pivotal for PGC1α-dependent renal stress resistanceJCI insightMarch 14, 2019Lynch MR, Tran MT, Ralto KM, Zsengeller ZK, Raman V, Bhasin SS, Sun N, Chen X, Brown D, Rovira II, et al.were incubated overnight at 4oC in 1:1500 dilution of rabbit polyclonal anti-nitrotyrosine antibody (A-21285 IgG2b; Thermo Fisher). Slides were then rinsed in PBS and developed using ImmPRESS HRP anti-rabbit IgG

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Nitrotyrosine Antibody from Thermo Fisher Scientific

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Nitrotyrosine Antibody from Thermo Fisher Scientific

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