Fig 1: RNAscope assays for MFSmod markers.(A) Feature plots showing expression of genes that are highly enriched in MFSmod cells (highlighted by broken-line circle) and significantly less active in other EC and SMC subpopulations of Fbn1mgR/mgR aortas. Thbs1, thrombospondin-1; Lum, lumican; Dcn, decorin; Mmp2, matrix metalloproteinase 2. (B) Dot plots showing expression of the ECM-related genes in each cell subpopulation of Fbn1mgR/mgR aortas. Dot size corresponds to the percentage of cells expressing each gene, and dot color corresponds to the level of expression. (C) Representative images of Hart’s staining of elastin in WT and Fbn1mgR/mgR aortas (top) and in situ RNAscope hybridization (below) using probes specific for Thbs1, Lum, Dcn, and Mmp2 in both WT and MFS (n = 3 per genotype). Scale bar: 50 μm. Asterisks indicate the lumenal side.