Fig 1: Effect of p120-catenin knockdown on EMT and Osimertinib resistance in H358OR cells by immunoblotting. (A) A total of 3.5 × 105 cells/well were seeded in a 6-well plate in antibiotic-free media until they achieved 80% confluency. The cells were then treated with either mock or p120-catenin siRNA and lysates were collected after 24, 48 and 72 h to perform immunoblotting. (B) The graph represents the fold change after immunoblotting of EMT-related proteins (p120-catenin and E-cadherin) after siRNA knockdown of p120-catenin. The modulations in the protein expression were calculated by densitometric analysis with respect to the parental cell lines using the ImageJ software and the results were statistically significant by two-tailed t-test analysis (n = 2, p < 0.05). (C) A total of 5500 cells/well were seeded in a 96-well plate in antibiotic-free media a day before transfection. The cells were then transfected with either mock or p120-catenin siRNA for 24 h and further treated with Osimertinib (10 µM) for 24 h. The percentage cell viability was analyzed with respect to mock siRNA transfection and treatment with Osimertinib compared to the combination of p120 siRNA with Osimertinib treatment and results were found to be statistically significant by two-tailed t-test analysis in both comparisons (n = 2, p < 0.05).