Western blot analysis of Mitofilin in isolated mitochondria using a Mitofilin Monoclonal antibody (Product # 45-6400) at a concentration of 2 µg/mL. Lane 1: Isolated mitochondria from Human heart at 5 µg, Lane 2: Isolated mitochondria from Bovine heart at 1 µg, Lane 3: Isolated mitochondria from Rat heart at 10 µg, Lane 4: Isolated mitochondria from Mouse heart at 10 µg, Lane 5: HepG2 cell lysate at 20 µg. Predicted band size: 84 kDa. Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.