Fig 2: Prognostic gene signatures related to breast cancer and ferroptosis. (A) A LASSO regression further screened prognosis-related genes from the ferroptosis-related significantly different genes (SDGs). (B) LASSO Cox regression models identified five prognostic ferroptosis-related SDGs for BC. (C) The expression levels of ALB were lower in tumor issues. (D) The expression levels of ANGPTL7 were lower in tumor issues. (E) The expression levels of BLOC1S5-TXNDC5 were higher in tumor issues. (F) The expression levels of IL6 were lower in tumor issues. (G) The expression levels of NGB were lower in tumor issues.

Fig 3: NGB is downregulated due to promoter methylation in CRC A The mRNA expression and promoter methylation of NGB in COAD. COAD: colon adenocarcinoma, data from UALACN (http://ualcan.path.uab.edu/). B Protein level of NGB in paired human colorectal carcinoma tissues detected by IHC staining, N = 3. C Fold-change of NGB mRNA expression detected by qRT-PCR in CRC and CRC-A. CRC: CRC, CRC-A: CRC-adjacent. D RT-PCR and Western blot results showing mRNA expression of NGB in CRC cell lines. E Expression of NGB in normal colon, primary tumor, liver metastasis and lung metastasis of CRC, data from GSE412584. F Methylated analysis of NGB in CRC cell lines, p < 0.05. G The mRNA expression level of NGB detected by qRT-PCR after treatment with demethylating agent Aza. H Methylation status of NGB detected by qMSP after treatment with demethylating agent Aza. All experiments were performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001