Fig 1: (a) Schematic showing the process of fibroblast-derived matrices and overview of experimental design. (b) Immunofluorescence imaging illustrating the lung, lymph, and mammary fibroblast matrices and deposited matrix, which is left intact post-lysis (visualised with Nidogen 1, green). Fibroblasts stained for actin (red). (c) Results of quantitative mass spectrometry, filtered for matrisome, of lung vs. mammary fibroblast-derived matrix (–log10 p-value 1.3 indicates proteins significantly higher in each condition). Venn diagram displays which lung and mammary ECM proteins were detected in those conditions. (d) Comparison of ECM proteins between lung and mammary fibroblast-derived matrix, and lung and mammary decellularised organs using the ISDoT method. Simple linear regression of data shows R2 value of 0.22. Venn diagram shows that 77 ECM proteins were detected in both data sets, 29 ECM proteins were only detected in the FDMs, and 146 proteins in decellularised organs.