Fig 2: Effects of miR-24-3p on protein expression of AV9L. (A) Protein expression level of AVL9 in human NSCLC tissues and paired-paracancerous tissues was determined via immunoblotting, (n=20/group). (B) Semi-quantitative analysis of the protein expression level of AVL9 in panel A. (C) Protein expression level of AVL9 in NSCLC cells, including H292, A549, H1299 and H460, and HBE cells, as detected by western blotting. (D) The binding sites between miR-24-3p and circ_0058357 were identified and shown in the top part of this panel. A549 and H1299 cells were co-transfected with mimic NC/miR-24-3p mimic and WT/MUT-AVL9, then the luciferase activity of AVL9 was detected and normalized with Renilla. (E) A549 and H1299 cells were firstly transfected with mimic NC or miR-24-3p mimic. Then, the protein expression level of AVL9 was evaluated via western blotting in the two cell lines. **P<0.01. NC, negative control; miR, microRNA; AVL9, AVL9 cell migration associated; NSCLC, non-small cell lung cancer; WT, wild-type; MUT, mutant; UTR, untranslated region.