Purified anti-beta-actin 643802 from BioLegend

Product Specs
ItemPurified anti-beta-actin
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number643802
Clone2F1-1
Conjugate/TagUnconjugated
Quantity100 µg
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
HostMouse
IsotypeIgG2b, Kappa
ReactivityHuman, Mouse, Rat
ApplicationsWestern Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)-P
Antibody TypePrimary
NCBI Gene AliasesActx, E430023M04Rik, beta-actin
NCBI Full Gene Nameactin, beta
Concentration0.50 mg/mL
ClonalityMonoclonal
Targetbeta-actin

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Expression of sirtuin 2 and 7 in placenta accreta spectrumRev Assoc Med Bras (1992)August 14, 2023Irmak Icen Taskin, Sevim Gurbuz, Mehmet Sait Icen, Dilek Cam Derin, Fatih Mehmet Findik(STJ25534) and anti-SIRT7 antibody (STJ25536) for 2 h at room temperature, and β-actin (Mouse IgG2b-643802-Biolegend) was used as a loading control. Appropriate HRP-conjugated secondary antibodies (Biolegend)

(2) Tumor-localized catalases can fail to alter tumor growth and transcriptional profiles in subcutaneous syngeneic mouse tumor modelsRedox BiologyAugust 1, 2023Allison Sheen, Yash Agarwal, Keith M. Cheah, Sarah C. Cowles, Jordan A. Stinson, Joseph R. Palmeri, Hadley D. Sikes, K. Dane Wittrupnitrocellulose, and probed with anti-catalase (1:500, Biolegend-863301), anti-beta-actin (1:500, Biolegend 643801), and/or anti-phosphotyrosine (1:1000, Abcam 179530) antibodies. Proteins were visualized using IRDye800CW

(3) Reprogramming and redifferentiation of mucosal-associated invariant T cells reveal tumor inhibitory activityeLifeApril 5, 2022Chie Sugimoto, Yukie Murakami, Eisuke Ishii, Hiroyoshi Fujita, Hiroshi Wakaoµg/ml) Antibody,"Anti-human/mouse/rat β-actin (2F1-1), purified (mouse monoclonal)",BioLegend,Cat# 643802; RRID:AB_2223199,Western blotting (1:2000 in 5 ml reaction) Antibody,"Anti-human/mouse LAT (11B.12),

(4) Rad52 mediates class-switch DNA recombination to IgDNature communicationsFebruary 21, 2022Xu Y, Zhou H, Post G, Zan H, Casali P.anti-phospho-Rad52 Ab (Y408472, Applied Biological Materials Inc.) or anti-β-Actin mAb (clone 2F1-1, 643802, BioLegend), the membranes were incubated with appropriate horseradish peroxidase (HRP)-conjugated secondary

(5) Effects of swimming training on myocardial protection in ratsBiomedical ReportsJanuary 31, 2022Chang-Chi Lai, Chia-Yu Tang, Szu-Kai Fu, Wei-Chin Tseng, Kuo-Wei Tsengchemiluminescence substrate system was used, followed by exposing the membranes to hyperfilms. β-actin (cat. no. 643802; BioLegend; 1:1,000) was used at room temperature for 2 h as a loading control. ImageJ version 1.6 (National

(6) Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuitsCommunications biologyJanuary 11, 2022Rodriguez E, Boelaars K, Brown K, Madunić K, van Ee T, Dijk F, Verheij J, Li RJE, Schetters STT, Meijer LL, et al.,R&D Systems,AF7174-SP,1:200 Polyclonal anti-human ZEB1,Atlas Antibodies,HPA027524,1:100 Purified anti-β-actin Antibody,Biolegend,643802,1:1000 Goat anti-rabbit - HRP,Dako,P0448,1:2000 Rabbit anti-mouse - HRP,Dako,P0161,1:1500 Goat anti-sheep

(7) Critical role of mitochondrial aldehyde dehydrogenase 2 in acrolein sequestering in rat spinal cord injuryNeural Regeneration ResearchJanuary 1, 2022Seth A. Herr, Liangqin Herr, Liangqin Gianaris, Yucheng Gianaris, Yucheng Jiao, Siyuan Race, Scott Shapiro, Scott Shapiro1:1000 rabbit anti-ALDH2 (Cat# PA5-11483, Thermo Fisher Scientific), 1:1000 rabbit anti-beta-actin (Cat# 643801, BioLegend). After overnight incubation, the membranes were further incubated with either biotinylated

(8) Divergent genetic regulation of nitric oxide production between C57BL/6J and wild-derived PWD/PhJ mice controls post-activation mitochondrial metabolism, cell survival, and bacterial resistance in dendritic cells.The Journal of ImmunologyJanuary 1, 2022Julia P. Snyder, Soyeon K. Gullickson, Roxana del Rio-Guerra, Andrea Sweezy, Bay Vagher, Tyler C. Hogan, Karolyn G. Lahue, Julie A. Reisz, Angelo D’Alessandro, Dimitry N. Krementsov, et al.Overnight incubations in primary antibodies iNOS (Cell Signaling #2982S, 1:1000) and β-Actin (BioLegend #643802, 1:2000) were followed by washing with TBST and then 1 hour incubations in secondary antibodies HRP

(9) Induction of neutrophil extracellular traps by Campylobacter jejuniCellular microbiologyAugust 1, 2020Callahan S, Doster RS, Jackson JW, Kelley BR, Gaddy JA, Johnson JG.anti-human Cit-H3 (Novus, Cat. NB100–57135SS) and 1:500 dilution mouse anti-human β-actin (Biolegend, Cat. 643801) were incubated on individual membranes for 1 hour at room temperature with shaking. Membranes were

(10) Targeted tumour theranostics in mice via carbon quantum dots structurally mimicking large amino acidsNature Biomedical EngineeringJuly 1, 2020Shuhua Li et al.data Antibodies Antibodies used LAT1, Cell signaling, Cat.# 5347S, 1:1000, lot: 3. beta Actin, Biolegend, Cat.# 643802, lot: B266259 Validation We confirmed each primary antibody for the species and application specified

(11) Syk-dependent glycolytic reprogramming in dendritic cells regulates IL-1β production to β-glucan ligands in a TLR-independent mannerJournal of leukocyte biologyDecember 1, 2019Thwe PM, Fritz DI, Snyder JP, Smith PR, Curtis KD, O'Donnell A, Galasso NA, Sepaniac LA, Adamik BJ, Hoyt LR, et al.C67E7), cleaved caspase‐1 (clone E2G2I), and cleaved IL‐1β (clone E7V2A) except for β‐actin (clone 643802), which was purchased from Biolegend. PI3K (20 uM), Syk (10 uM), and TBK1 (5 uM) pharmacologic inhibitors

(12) Bovine κ-Casein Fragment Induces Hypo-Responsive M2-Like Macrophage PhenotypeNutrientsJuly 23, 2019Lalor R, O'Neill S.(IκBα, No. ab32518) antibody was purchased from Abcam (Cambridge, UK). Mouse monoclonal β-actin (No. 643802) antibody was purchased from Biolegend (San Diego, CA, USA). Horseradish peroxidase-conjugated anti-species

(13) Long intergenic non-coding RNAs regulate human lung fibroblast function: Implications for idiopathic pulmonary fibrosisScientific reportsApril 15, 2019Hadjicharalambous MR, Roux BT, Csomor E, Feghali-Bostwick CA, Murray LA, Clarke DL, Lindsay MA.(Tris-buffered saline with Tween) blocking buffer with the following antibodies: β-actin (BioLegend, 643802), H3K4me1 (Diagenode, C15410194), H3K4me3 (Diagenode, C15410003) and H3K27ac (Diagenode, C15410196).

(14) Anti-edema and antioxidant combination therapy for ischemic stroke via glyburide-loaded betulinic acid nanoparticlesTheranosticsJanuary 1, 2019Deng G, Ma C, Zhao H, Zhang S, Liu J, Liu F, Chen Z, Chen AT, Yang X, Avery J, et al.29, using antibodies targeting Nrf2 (Novus Biologicals), HO-1 (Novus Biologicals), and beta-actin (#643802, BioLegend). To determine the anti-oxidant effect in vivo, mice with successful MCAO surgery were randomly

(15) Regulation of Oxidative Stress in Corneal Endothelial Cells by Prdx6Antioxidants (Basel, Switzerland)December 4, 2018Lovatt M, Adnan K, Peh GSL, Mehta JS.

(16) Epidermal fatty acid binding protein prevents chemical-induced skin tumorigenesis by regulation of TPA-induced IFN/p53/SOX2 pathway in keratinocytesJournal of Investigative DermatologySeptember 1, 2018Yuwen Zhang et al.Santa Cruz Biotech), anti-E-FABP antibody (cat#AF1476, R&D systems), and anti-β-actin antibody (2F1-1, cat#643802, Biolegend), were used for overnight incubation (binding). For analysis of p53 expression in primary

(17) The 17,18-epoxyeicosatetraenoic acid-G protein-coupled receptor 40 axis ameliorates contact hypersensitivity by inhibiting neutrophil mobility in mice and cynomolgus macaquesThe Journal of allergy and clinical immunologyAugust 1, 2018Nagatake T, Shiogama Y, Inoue A, Kikuta J, Honda T, Tiwari P, Kishi T, Yanagisawa A, Isobe Y, Matsumoto N, et al.

(18) Discovery of LSZ102, a Potent, Orally Bioavailable Selective Estrogen Receptor Degrader (SERD) for the Treatment of Estrogen Receptor Positive Breast CancerJournal of Medicinal ChemistryApril 12, 2018George S. Tria et al.antibody (Santa Cruz Biotechnology, HC-20), as well as an anti-β-actin antibody (BioLegend, clone 2F1-1 cat. no. 643801) as a loading control. Western blots were scanned into (Bio Rad ChemiDoc Imaging Systems) for quantiﬁcation

(19) Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatmentOncology lettersFebruary 1, 2018Qin S, Gao Z, Liu Y, Liu C, Wang J, Zou LL.respectively). Purified anti-β-actin antibody was purchased from Biolegend, Inc. (San Diego, CA, USA; cat. no. 643801). Horseradish peroxidase (HRP)-labeled goat anti-mouse immunoglobulin G (IgG) [heavy and light (H+L)]

(20) Herpes simplex virus 1 interferes with autophagy of murine dendritic cells and impairs their ability to stimulate CD8⁺ T lymphocytesEuropean journal of immunologyOctober 1, 2017Budida R, Stankov MV, Döhner K, Buch A, Panayotova-Dimitrova D, Tappe KA, Pohlmann A, Sodeik B, Behrens GMN.

Be the First to Write a Review!

Purified anti-beta-actin from BioLegend

Description

BioLegend

Citations (30)

Reviews

Purified anti-beta-actin from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map