Fig 2: Transcriptome sequencing analysis of platelet activation following PHB2 knockdown. (A) Transcriptome of the screened differentially expressed genes. (B) Venn diagram of the differentially expressed genes between the NC and shPHB2 groups. NC, negative control; sh, short hairpin RNA; PHB2, prohibitin 2.

Fig 3: PHB2 knockdown in platelets. PHB2 (A) mRNA and (B) protein expression levels in platelets were analyzed via reverse transcription-quantitative PCR and western blotting, respectively. *P<0.05. PHB2, prohibitin 2; NC, negative control; RNAi, interference RNA.

Fig 4: Effect of PHB2 on platelet mitophagy. (A) LC3 protein expression levels were measured via western blotting. (B) Flow cytometry was used to determine the ??m in platelets. *P<0.05, **P<0.01 and ***P<0.001. ??m, mitochondrial membrane potential; NC, negative control; sh, short hairpin RNA; CCCP, chlorophenylhydrazone.

Fig 5: Analysis of activated genes affected by PHB2 in platelet KEGG enrichment. Kyoto Encyclopedia of Genes and Genomes signaling pathway enrichment analysis of the effect of differentially expressed genes on the platelet activation pathway.