Fig 1: SRSF4 is a mechanosensitive RBP.Differentiated muscle cells were stretched for 1 h or 3 h and western blot assays were performed and quantified by densitometry to examine total and phosphorylated levels of SRSF4 (a), SRSF5 (b), and SRSF6 (c). no: non-stretched samples. s: stretched samples. d Some overlapping genes and splicing events from the SRSF4 pie charts shown in Fig. 6c were depicted in cartoons for 1 h and 3 h stretching time points showing how mechanosensitive targets and those regulated by SRSF4 might play a role in the MAPK signaling cascade. ATF3 activating transcription factor 3, AREG amphiregulin, BMF Bcl2 modifying factor, CYR61 cysteine-rich angiogenic inducer 61, CYP1A1 cytochrome p450 family 1 subfamily A member 1, CTGF connective tissue growth factor, DDR1 discoidin domain receptor tyrosine kinase 1, DUSP1 dual specificity phosphatase 1, DUSP5 dual specificity phosphatase 5, EGFR epidermal growth factor receptor, ERK extracellular signal-regulated kinase, EREG epiregulin, FGFR3 fibroblast growth factor receptor 3, FHL2 four and a half LIM domains 2, FOS Fos proto-oncogene AP-1 transcription factor subunit, FOSL1 FOS like 1 AP-1 transcription factor subunit, GPR3 G-protein coupled receptor 3, GPR39 G-protein coupled receptor 39, HBEGF heparin binding EGF like growth factor, LATS large tumor suppressor kinase 1, JNK c-Jun N-terminal kinase, MADD MAP kinase activating death domain, MEIS meis homeobox 1, MYC MYC proto-oncogene, NPR3 natriuretic peptide receptor 3, PBX3 PBX homeobox 3, PIT1 pituitary transcript factor 1, PLAU plasminogen activator urokinase, PTPN22 protein tyrosine phosphatase non-receptor type 22, P38 p38 mitogen-activated protein kinase, RGS2 regulator of G-protein signaling 2, SQLE squalene epoxidase, TEAD4 TEA-domain transcription factor 4, TIMP3 TIMP metallopeptidase inhibitor 3, YAP yes-associated protein. Results are shown as mean ± SEM, N = 4 (a), N = 4 (b), N = 4 (c) *p < 0.05, Welch’s T-test.