Fig 1: Molecular models of the mouse P2X7 subunits with amino acid residue Pro451 or 451Leu. (A–D) The full-length mouse P2X7 subunit (NCBI) was modeled with the I-TASSER protein structure prediction suite [142,143] using the cryo-electron microscopy structure of rat P2X7, PDB ID: 6u9v [84] as a template. Sections of the P2X7 subunits are colored as depicted by the dolphin-like structure (inset) of the P2X4 subunit [144], including the head (blue), upper body (violet), right (magenta) and left (yellow) flippers, dorsal fin (red), lower body (gray), transmembrane domain-spanning fluke (green), and the intracellular ballast domain (not shown on inset; white). Boxed areas indicate the region containing the Pro451Leu SNP. (A) Modeling of P2X7-Pro451 returned a C-score of 0.25 with an estimated TM-score of 0.75 ± 0.11 and root mean square deviation of 7.1 ± 4.2 Å. (B) Close-up images of boxed areas, with Pro451 shown as ball and stick structures (magenta). Amino acid residues within 5 Å of the residue of interest are also labeled (cyan). (C) Modeling of P2X7-451Leu returned a C-score of 0.20 with an estimated TM-score of 0.74 ± 0.11 and root mean square deviation of 7.2 ± 4.2 Å. (D) Close-up images of boxed areas, with Leu451 shown as ball and stick structures (magenta). Amino acid residues within 5 Å of the residue of interest are also labeled (cyan). Images were produced using Mol* [145]. Figure created in BioRender.com with permission.