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Pacific Blue(TM) Annexin V
Pacific Blue(TM) Annexin V from BioLegend
(1)
Write a review
Citations:
(59)
Supplier Page
Supplier Page from
BioLegend for
Pacific Blue(TM) Annexin V
Description
Pacific Blue(TM) Annexin V
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Pacific Blue(TM) Annexin V
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
640917
Quantity
25 tests
Applications
Flow Cytometry (FC)
Conjugate/Tag
Pacific Blue
NCBI Gene Aliases
ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
NCBI Full Gene Name
annexin A5
Format
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Target
Annexin V
Species
Human, Mouse, Rat
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (59)
(1)
AMPK activation induces immunogenic cell death in AML
Blood Advances
December 26, 2023
Johanna Mondesir, Margherita Ghisi, Laura Poillet, Robert A. Bossong, Oliver Kepp, Guido Kroemer, Jean-Emmanuel Sarry, Jérôme Tamburini, Andrew A. Lane
ab196159
); allophycocyanin anti-human CD45 (
BioLegend
, no.
368512
); annexin V–Pacific Blue (
BioLegend
, no.
640918
); allophycocyanin anti-mouse CD11c (
BioLegend
, no.
117310
); Brilliant
Violet
510 anti-mouse CD86 (
BioLegend
,
(2)
Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines
BIO-PROTOCOL
July 20, 2023
Kateryna Shkarina, Petr Broz
number:
G8741
)DRAQ7 (
BioLegend
, catalog number:
424001
)Annexin V Pacific Blue (BioLegend, catalog number:
640918
)Annexin V FITC (
BioLegend
, catalog number:
640906
)Annexin V Alexa Fluor 647 (
BioLegend
, catalog number:
(3)
The DREAM complex functions as conserved master regulator of somatic DNA-repair capacities
Nature Structural & Molecular Biology
April 1, 2023
Arturo Bujarrabal-Dueso, Georg Sendtner, David H. Meyer, Georgia Chatzinikolaou, Kalliopi Stratigi, George A. Garinis, Björn Schumacher
were incubated in
annexin V binding buffer
(
BioLegend
,
422201
) with Pacific Blue annexin V (
BioLegend
,
640917
) and 7-AAD (
Thermo Fisher Scientific
,
00699350
) at 4 °C for 15 min. Cells were measured using a
MACSQuant VYB
(4)
Inhibition of Autophagy Promotes the Elimination of Liver Cancer Stem Cells by CD133 Aptamer-Targeted Delivery of Doxorubicin
Biomolecules
November 3, 2022
Wang Yin, Cuong V. Pham, Tao Wang, Hadi Al Shamaileh, Rocky Chowdhury, Shweta Patel, Yong Li, Lingxue Kong, Yingchu Hou, Yimin Zhu,
et al.
apoptosis of cells. The cells were trypsinized and stained with annexin V (
BioLegend
, San Diego, CA, USA; #
640918
,
1:20
dilution) and 7-AAD (
BioLegend
, San Diego, CA, USA; #
420404
,
1:20
dilution) for 15 min at room
(5)
Alternative splicing of apoptosis genes promotes human T cell survival
eLife
October 20, 2022
Davia Blake, Caleb M Radens, Max B Ferretti, Matthew R Gazzara, Kristen W Lynch
flow cytometry analysis
. Cell
survival analysis
was measured by
flow cytometry
through Annexin V-Pacific Blue (
Biolegend
:
640917
) and
Propidium Iodide
staining
(
Thermo Fisher
:
c34557
).
Caspase-3
/7 activity was measured with FAM-FLICA
(6)
Functional requirements for a Samd14-capping protein complex in stress erythropoiesis
eLife
June 17, 2022
Suhita Ray, Linda Chee, Yichao Zhou, Meg A Schaefer, Michael J Naldrett, Sophie Alvarez, Nicholas T Woods, Kyle J Hewitt
like PE–
CD71
(R17217), APC-
Ter119
(
116212
), PE-Cy7- Kit (
clone
2B8) (
105814
), Pacific Blue Annexin V (
640917
) antibodies for 30 min at 4°C (all from
Biolegend
). Cells were resuspended with either
DAPI
or Draq7
(7)
T cell stimulation remodels the latently HIV-1 infected cell population by differential activation of proviral chromatin
PLOS Pathogens
June 6, 2022
Birgitta Lindqvist, Bianca B. Jütte, Luca Love, Wlaa Assi, Julie Roux, Anders Sönnerborg, Tugsan Tezil, Eric Verdin, J. Peter Svensson
(50 ng/sample,
Biolegend
, Cat#
421301
) and Annexin V (5 μl, Pacific Blue conjugated,
Biolegend
, Cat#
640917
) were added and the cells were incubated for 15 min at 4°C in the dark before analysis on a Cytoflex
(8)
Combined Inactivation of CTPS1 and ATR Is Synthetically Lethal to MYC-Overexpressing Cancer Cells
Cancer research
March 15, 2022
Sun Z, Zhang Z, Wang QQ, Liu JL.
instructions. Annexin V/propidium iodide (PI)
staining
was used to determine apoptosis. Pacific Blue Annexin V (
640918
) was purchased from
Biolegend
. PI (ST511) was purchased from Beyotime. Annexin V/PI
staining
was performed
(9)
Zinc finger protein Zfp335 controls early T-cell development and survival through β-selection-dependent and -independent mechanisms
eLife
February 3, 2022
Wang X, Jiao A, Sun L, Li W, Yang B, Su Y, Ding R, Zhang C, Liu H, Yang X,
et al
.
109814
; RRID:AB_389211 Antibody,anti-mouse CD3 Pacific Blue (
Armenian Hamster
monoclonal),
Biolegend
,Cat#
640918
; RRID:AB_493645,Cat#
640918
; RRID:AB_493645 Antibody,anti-mouse CD27 FITC (
Armenian Hamster
monoclonal),
Biolegend
,Cat#
(10)
The inhibition of ABCB1/MDR1 or ABCG2/BCRP enables doxorubicin to eliminate liver cancer stem cells
Scientific Reports
December 1, 2021
Wang Yin et al.
(
1:20
dilution), VioBright FITC CD133/1 antibody (
1:50
dilution), Pacific Blue Annexin V (
Biolegend
, #
640918
,
1:20
dilution), and 7-AAD (
Biolegend
, #
420404
,
1:20
dilution) for 15 min at room temperature in the
(11)
Pannexin 1 channels facilitate communication between T cells to restrict the severity of airway inflammation
Immunity
August 10, 2021
Medina CB, Chiu YH, Stremska ME, Lucas CD, Poon I, Tung KS, Elliott MR, Desai B, Lorenz UM, Bayliss DA,
et al
.
Corp.,Cat# LS004176 7AAD Viability Stain,
Thermo Fisher
,Cat#
A1310
Annexin V Pacific Blue,
Biolegend
,Cat#
640918
EdU,
Thermo Fisher
,Cat#
A10044
Protease Inhibitors
,Calbiochem,Cat# 539131 CFSE,
Invitrogen
,Cat#
C34554
ATP
,
Sigma-Aldrich
,Cat#
(12)
Tumor regression and immunity in combination therapy with anti-CEA chimeric antigen receptor T cells and anti-CEA-IL2 immunocytokine
OncoImmunology
April 2, 2021
Seung E. Cha, Maciej Kujawski, Paul J. Yazaki, Christine Brown, John E. Shively
APC-conjugated antibody (R&D, FAB412181A).
Mouse
cells were stained with Pacific Blue Annexin V (
Biolegend
,
640918
) or Horizon
Fixable Viability Stain 510
(
BD
,
564406
) for cell viability.
Flow cytometry
was performed
(13)
In oxygen-deprived tumor cells ERp57 provides radioprotection and ensures proliferation via c-Myc, PLK1 and the AKT pathway
Scientific reports
March 30, 2021
Ocklenburg T, Neumann F, Wolf A, Vogel J, Göpelt K, Baumann M, Baumann J, Kranz P, Metzen E, Brockmeier U.
× 105 cells were incubated with 80 µg/ml PI and 9.6 µg/ml Annexin V conjugated to PacificBlue (No.
640917
,
Biolegend
, San Diego, CA, USA) in
Annexin V binding buffer
(No.
422201
,
Biolegend
) for 15 min at room
(14)
Erythropoietin Promotes Infection Resolution and Lowers Antibiotic Requirements in
E. coli-
and
S. aureus
-Initiated Infections
Frontiers in immunology
January 1, 2021
Liang F, Guan H, Li W, Zhang X, Liu T, Liu Y, Mei J, Jiang C, Zhang F, Luo B,
et al
.
(
406421
[Poly4064];
BioLegend
, San Diego, CA, USA), p-Jak2 (
Ab219728
[E132];
Abcam
, Cambridge, UK), annexin V (
640918
;
BioLegend
, San Diego, CA, USA),
E. coli
antibody (
GTX40856
[polyclonal];
GeneTex
, Irvine, CA, USA),
(15)
Obesity induces gut microbiota alterations and augments acute graft-versus-host disease after allogeneic stem cell transplantation
Science Translational Medicine
November 25, 2020
Lam T. Khuat et al.
single-cell suspensions (1 – 5 × 106 cells/ml were stained with Annexin V-Pacific Blue (
Biolegend
Cat#
640918
, San Diego, CA, USA). Cells then were incubated in dark for 15 minutes and stained with PI (Life Technologies,
(16)
EZH2
-Deficient T-cell Acute Lymphoblastic Leukemia Is Sensitized to CHK1 Inhibition through Enhanced Replication Stress
Cancer Discovery
July 1, 2020
Theresa E. León et al.
(control), cells were collected and washed with PBS, stained using Pacific Blue-Annexin V (
BioLegend
; Cat.
640917
) and analyzed by
flow cytometry
using a BD FACSymphonyTM cytometer (BD Biosciences). 10,000 events were
(17)
Diaphanous-related formin mDia2 regulates beta2 integrins to control hematopoietic stem and progenitor cell engraftment
Nature communications
June 23, 2020
Mei Y, Han X, Liu Y, Yang J, Sumagin R, Ji P.
above, followed by Cy5-Annexin V (Catalog #
559934
,
BD
Pharmingen) or Pacific blue-Annexin V (Catalog #
640918
,
BioLegend
)
staining
(
1:200
diluted in
Annexin V-binding buffer
) at room temperature for 15–20 min.
(18)
STING Gain-of-Function Disrupts Lymph Node Organogenesis and Innate Lymphoid Cell Development in Mice
Cell reports
June 16, 2020
Bennion BG, Croft CA, Ai TL, Qian W, Menos AM, Miner CA, Frémond ML, Doisne JM, Andhey PS, Platt DJ,
et al
.
Recombinant murine FLT3-ligand
,
Peprotech
,Cat#
250-31L
Promega
RNasin Plus Rnase Inhibitor
,
Promega
,Cat#
N2611
Annexin V,
Biolegend
,Cat#
640917
Annexin V,
BD Biosciences
,Cat#
556421
Critical Commercial
Assays
,, Mouse IgA ELISA Kit,"Immunology
(19)
Disruption of TFIIH activities generates a stress gene expression response and reveals possible new targets against cancer
Open biology
June 1, 2020
Uriostegui-Arcos M, Aguayo-Ortiz R, Valencia-Morales MDP, Melchy-Pérez E, Rosenstein Y, Dominguez L, Zurita M.
(Invitrogen, Cat. 65-0865) and Biolegend Pacific Blue Annexin V or
FITC Annexin V
(
BioLegend
, Cat.
640918
or Cat.
640906
, respectively). Intracellular
protein staining
was performed as previously described
(20)
Mitochondrial Pyruvate Carrier 1 Promotes Peripheral T Cell Homeostasis through Metabolic Regulation of Thymic Development
Cell reports
March 3, 2020
Ramstead AG, Wallace JA, Lee SH, Bauer KM, Tang WW, Ekiz HA, Lane TE, Cluntun AA, Bettini ML, Round JL,
et al
.
H1.2F3,
Biolegend
,Cat#
104508
Rat anti-mouse CD62L
Clone
MEL-14,
Biolegend
,Cat#
104412
Annexin V,
Biolegend
,Cat#
640918
7-AAD Viability Staining Solution
,
Biolegend
,Cat#
420404
Rat anti-mouse CD117
Clone
ACK2,
Biolegend
,Cat#
135106
Ultra-LEAF
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Reviews
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(1)
1/23/2019
Pacific Blue Annexin V
Anup Kumar
I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This antibody is good for detecting Annexin V on mouse immune cells to ...
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Pacific Blue(TM) Annexin V from BioLegend
Supplier Page
View Company Product Page
Pricing Info
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