Fig 1: Gating strategy for skin ILCs and myeloid cells(A) The gating strategy for ILCs. Lineage markers include CD3e, CD5, CD19, CD11b, Cd11c, FceRIa, NK1.1 and CD2. Lin+ Thy1– cells are myeloid cells. The majority of Lin+ Thy1+ cells are T cells. Lineage– Thy1+ cells are identified as a mixture of ILC2s and ILC3s. From this gate, GATA3high cells are ILC2 and GATA3low ROR?thigh cells are ILC3s.(B) The gating strategy of skin myeloid cells. Lineage markers include CD3e, CD19, NK1.1 and Siglec-F. Gate for CD45+ lineage– (Siglec-F–, CD3e–, CD19– and NK1.1–) to exclude eosinophils, T cells, B cells and NK cells. Lineage– CD11b+ Ly6c+ cells are identified as monocytes. From the CD11b+ Ly6c– gate, macrophages (EpCAM– CD64+), Langerhans cells (CD64– EpCAM+ MHC II+) and dermal dendritic cells (CD64– EpCAM– MHC II+ CD11c+) are identified.