Fig 1: Histology of the mesenteric arteries in Nppb-/- at 4 and 9 months.(A) H&E analysis of progressive mesenteric arterial lesions in Nppb-/- . Mesenteric tissues obtained from animals of early and late symptom onset; 4 and 9 months in Nppb-/- vs 9 month Nppb+/+ (Control arteries highlighted by white arrows). (B) Factors involved in Nppb-/- mesenteric arterial remodeling: representative immunofluorescent microphotographs from 9 month Nppb-/- (n = 4), 4 month Nppb-/- (n = 3), and 9 month Nppb+/+(n = 3) sections, out of an average of 5 images per animal/section of mesenteric sections are shown. Specific antibodies include anti-Fgf2 (red), anti-Desmin (red), anti-a-SMA (green), anti-IL6 (green), anti-IL8 (green), and anti-CD45 (blue). Nuclei were counterstained by DAPI (white). SM; smooth muscle, ME: Mesentery, Control arteries highlighted by white arrows.
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