Fig 1: CYTOF Analysis of Key Mesenchymal Subset Markers Reveals Colitis-Associated Stromal Remodeling(A) CyTOF panel detected colonic mesenchymal populations. Stromal subsets are represented by indicated markers.(B) Heatmaps of selected markers on concatenated healthy and inflamed t-SNE plots representing key stromal subsets. Color maps by F3 (CD142), POSTN, IL-33, CCL19, BCL6, and PTGS2 shown.(C) Expansion of S4 in UC detected by scRNA-seq.(D) Histogram comparisons of CCL19 and TNFSF14 (LIGHT) levels in healthy versus inflamed colonic mesenchyme marks the emergence of S4.(E) t-SNE comparisons of healthy versus inflamed colonic mesenchyme. Clustering used the following parameters: F3/CD142, POSTN, PDGFRA, PDPN, BCL6, PTGS2, CD55, CCL19, CCL21, IL-33, LIGHT, CLU, FDCSP, and aSMA. Select markers representing S2 and S4 in healthy versus inflamed tissues shown.(F) Graphical summary of the most significantly changed markers in UC. Each dot represents one independent pair of healthy donor and patient samples.See also Figure S5.