Fig 1: Adhesion molecule phenotype of BLECs and HIBCPP cells. Cell surface staining of BLECs (a) and HIBCPP cells (b) for the adhesion molecules ICAM-1, ICAM-2, VCAM-1, P-selectin, E-selectin and CD99 was analyzed by flow cytometry. c Cell surface staining of BLECs for the P-selectin and von Willebrand factor (vWF) was analyzed by flow cytometry. Isotype control, non-stimulated (NS) and 16 h pro-inflammatory cytokine-stimulated condition (1 ng/mL TNF-α + 20 IU/mL IFN-γ) are represented respectively in orange, blue and red in a histogram overlay. Immunofluorescence staining on BLECs (e) and HIBCPP cells (d) for ICAM-1 (red), ICAM-2 (red), VCAM-1 (green), P-selectin (red), E-selectin (red) and CD99 (red). Nuclei were stained with DAPI (blue). Each staining is representative of at least 3 independent experiment performed on 3 distinct filters. Both, NS and 1 ng/mL TNF-α + 20 IU/mL IFN-γ stimulated conditions are shown. Scale bar = 50 μm