Fig 2: Accumulation of TIM4neg Macrophages in a Mouse Model of NASH(A) Body weight of C57BL/6 male mice fed a HFD for the indicated time.(B–D) Liver triglyceride (B) and mRNA markers of steatosis (C) and fibrosis (D) were quantified.(E) H&E (top panels) and picrosirius red (bottom panels) from liver tissue.(F) Flow-cytometry plots are shown for CD45+, live, singlets (top panels). Representative plots of F4/80hi cells (red gate) were analyzed for expression of TIM4 (bottom panels).(G) Quantification of flow data per gram of liver tissue.(H) Percentage of F4/80hi cells that are TIM4neg over course of HFD feeding.In (A), each dot represents the mean ± SEM and in (B)–(D). In (G), each dot represents a single mouse, and the mean is shown (n = 7–8 mice/group). The data represent the results of one independent experiment with n = 7–8 mice per group. *p < 0.05 for HFD versus STD. Scale bars, 250 µm