Fig 1: Levels of the ABC transporter proteins (P-gp, Bcrp, and Mrp1) in mouse brain micro vessels isolated from the cortex and the hippocampus. (A–C) The relative mRNA levels of abcb1a (A), abcg2 (B), and abcc1 (C) were assessed through real-time PCR. GAPDH was used as an internal standard. Data are represented as (2-?Ct). The protein expressions of P-gp (A), Bcrp (B), and Mrp-1 (C) were examined using Western blot analysis. Optical densities were analyzed with Image Lab 6.0.1 software (Bio-Rad) and normalized to GAPDH. Results are the means ± SEM from three lots of brain endothelial micro vessels (each lot represents a pool from five mice). Statistical comparisons: * p < 0.05.