Fig 1: Mutant KRAS cells treated with ethanol display a higher level of transcriptional and translational coordination. (A) Multiple Co-inertia analysis of RNA-Seq and proteomics data. Length of the line connecting the proteomics and RNA-Seq data represents their dissimilarity; RNA-Seq n = 1, proteomics n = 3. (B) Venn diagrams showing differentially expressed protein coding genes (RNA-Seq, ±2-fold change) and proteins (Proteomics, ±1.25-fold change) common in the RNA-Seq and proteomics data. (C) Heatmaps of all conditions showing expression of commonly detected up-(33) and down-(11) regulated protein-coding transcripts (left) and proteins (right) found in HPNE-KRAS EtOH cells compared to HPNE-KRAS cells. (D–I). Validation of omics data by qRT-PCR and Western blot for CD81 (D), RHOG (E), ZPR1 (F), RUVBL1 (G), PRPF19 (H), and NT5E (I); indicated in (C) by *. The qRT-PCR was performed to validate RNA-Seq expression with GAPDH used for normalization. Mean ± SEM; n = 4. Western blots were used to validate proteomics expression using HSC70 or GAPDH as loading control. Cluster of Differentiation 81 (CD81); Ras Homolog Family Member G (RHOG); ZPR1 zinc finger (ZPR1); RuvB like AAA ATPase 1 (RUVBL1); Pre-MRNA Processing Factor 19 (PRPF19); 5'-Nucleotidase (NT5E). The original blots of Figure 5 could be found in Figure S5.