Fig 1: Vav1 Is Downmodulated in TLX+ T-ALL(A) Heatmap of indicated mRNAs (left) in T-ALL dataset 1. The identification number (left) and molecular subtype of patients (top) are indicated. Signal log ratio abundance is depicted as in Figure 2A.(B) Scatterplot showing VAV1 expression across indicated human T-ALL subtypes (bottom) using microarray dataset 1. Dots represent values from an individual sample. Bars represent the mean expression value ± SEM for the overall sample set. ***p = 0.001 (Tukey's HSD test).(C) Scatterplot showing VAV1 abundance against the combined amount of TLX1/TLX3 expression using dataset 1. Dots represent values from individual samples.(D) ssGSEA-generated heatmap of the up- and downregulated genes of the “tumor-specific” Vav1-/-/Zfp36l1-/-;Zfp36l2-/- signature in T-ALL cases from dataset 1. ssGSEA enrichment scores are depicted on a dark blue (lowest) to dark red (highest) scale. Samples with moderate and high signature fits are highlighted by gray and black bars, respectively. TS, tumor-specific.(E) Box plot of the tumor-specific Vav1-/-/Zfp36l1-/-;Zfp36l2-/- gene signature fit score for indicated T-ALL subtype samples (bottom) and microarray datasets (top). Data are presented as indicated in Figure 2C. **p = 0.01, ***p = 0.001 (Tukey's HSD test).(F) Expression correlation matrix from TLX+ T-ALL samples positive for the “tumor-specific” Vav1-/-/Zfp36l1-/-;Zfp36l2-/- gene signature using dataset 1. Positive and negative correlation is shown in red and blue, respectively. The size of circles and color intensity are proportional to the Pearson correlation coefficient found for each transcript pair. Correlations with p values below the significance threshold of 0.05 (which relates with a Pearson correlation coefficient above 0.39 for dataset 1) have been considered as statistically significant and labeled with asterisks. Negative regulators of the Notch1 route and ICN1 targets are shown in red and blue letters, respectively.(G) Pearson correlation coefficient of the HES1 mRNA with indicated transcripts (inset) and microarray datasets (top). The horizontal blue broken lines depict the p-value threshold used (0.05) to consider a correlation statistically significant. TLXSP, TLX+ T-ALL signature positive; T-ALLSN, T-ALL signature negative.(H and I) Expression of endogenous Vav1 in TCLs from indicated T-ALL cell lines (H) and patient-derived tumor cells (I) (n = 3). TLX and HOXA status of cells is shown on top.See also Figure S7.