Fig 2: Immunohistochemical detection of ubiquitin D (UBD) and cyclin-dependent kinase 4 inhibitor D (CDKN2D) expression in left-sided colon carcinoma (LSCC) and right-sided colon carcinoma (RSCC). Immunohistochemical analyses were performed to determine (A and B) CDKN2D and (C and D) UBD expression in (A and C) LSCC and (B and D) RSCC. Sections were stained using the avidin-biotin complex method. The coloration was developed with 3,3′-diaminobenzidine (DAB), and the sections were then counterstained with hematoxylin. Positive staining for either CDKN2D or UBD appeared brown. Magnification, x400.

Fig 3: Western blot analysis of ubiquitin D (UBD) and cyclin-dependent kinase 4 inhibitor D (CDKN2D) expression in left-sided colon carcinoma (LSCC) and right-sided colon carcinoma (RSCC). (A) LSCC and RSCC tissue lysates were subject to western blot analysis for UBD and CDKN2D expression. β-actin blotting was used as a loading control. (B) UBD, CDKN2D and β-actin blots were measured by densitometry. The densities of the UBD and CDKN2D blots were normalized against that of β-actin, to obtain a relative UBD or CDKN2D blot density. *P<0.05 compared with LSCC.