Fig 1: METTL14 is increased in TNF-a-induced endothelial cells and atherosclerotic lesions. (A) QRT-PCR detection of METTL3, METTL14, KIAA1429, WTAP, FTO and ALKBH5 in HUVECs stimulated with 10 ng/ml TNF-a for 6 h or 12 h. (B) Representative western blot results of METTL3, METTL14, KIAA1429, WTAP, FTO and ALKBH5 after TNF-a stimulation in HUVECs. (C and D) Endothelial mononuclear adhesion assays in HUVECs after knockdown of METTL14 (with or without TNF-a stimulation) and the statistics of the relative monocyte adhesion rate. (E) QRT-PCR detection of VCAM-1, ICAM-1 and E-selectin in HUVECs stimulated with 10 ng/ml TNF-a for 12 h with or without METTL14 knockdown. (F) Representative western blot results of VCAM-1 and ICAM-1 in HUVECs stimulated with 10 ng/ml TNF-a for 12 h with or without METTL14 knockdown. (G) QRT-PCR detection of VCAM-1 and ICAM-1 in HUVECs with or without METTL14 knockdown or METTL14 overexpression. (H) Representative western blot results of VCAM-1 and ICAM-1 in HUVECs with or without METTL14 knockdown or METTL14 overexpression. (I and J) METTL14 immunostaining in atherosclerosis samples from APOE-/- mice and the statistics on the proportion of METTL14-positive cells. Non AS represents the normal vascular area near the atherosclerotic plaques. AS stands for the atherosclerotic plaque area. The white and yellow arrows indicate METTL14-positive endothelial cells in the AS plaque and Non AS area, respectively. (K) QRT-PCR detection of METTL14 mRNA in normal vascular tissues or atherosclerotic lesions collected from APOE knockout mice. (Data are presented as the mean ± SEM. A, D, E, and G, One-way ANOVA with Bonferroni's post hoc test was applied to compare the indicated groups. J and K, Two-tailed unpaired Student's t test was applied to compare the two groups. *P<0.05, **P<0.01, and ***P<0.001).