Fig 1: CCNYc/PFTK1 complex promoted the assembly of F-actin via TPM4. (A–C) F-actin was marked by IF staining. Bars represent 20 µm. (D) Expression level of TPM4 was shown in the figure (upper panel). The quantification of TPM4 level was done by gray analysis (lower panel). **p < 0.01. (E) TPM4 level in PFTK1 down-regulated H1299 CCNYc cells. Upper panel, the picture of WB, lower panel, the quantification of the TPM4 level. **p < 0.01 vs. H1299-CCNYc cells expressing control shRNA. (F) TPM4 level was detected by WB. The quantification of TPM4 was shown in the right panel. **p < 0.01. (G) F-actin was marked by phalloidin conjugating to TRITC. Bars represent 20 µm. (H) RhoA activity was regulated by CCNY. GTPase activity assay was used to determine the activated level of (GTP-) Rac1, RhoA, and Cdc42. The phosphorylated FAK on Y397, as well as the FAK level, were examined by immunoblotting. The protein levels were quantified by gray analysis and showed in the right panel. **p < 0.01 vs. H1299 cells transfected with pEGFP-N1 vector.