Fig 1: The mRNA expressions of NRG1 and ErbB. (A, B) Rats from group D had lower NRG1 and ErbB mRNA expression compared with groups C, DE and DM (all p < 0.01), while the ErbB mRNA expression of rats in group C was higher than groups DE (p < 0.01) and DM (p < 0.05). (C, D) The protein levels of NRG1 (p < 0.01) and ErbB4 (p < 0.05) were significantly lower in group D than group C NRG1 and ErbB protein levels were significantly higher in group DE (both p < 0.05 and very significantly higher in group DM (both p < 0.01) than those in group D n = 10 rats per group; *p < 0.05, **p < 0.01; C (control), D (diabetic), DE (diabetic + exercise), DM (diabetic + MOTS-c).
Fig 2: Expression of NRG1 (a) and ErbB2 (b) is demonstrated by Western blotting normalized to GAPDH. Panels above the graph are representative Western blot images from different groups. *P < 0.05 vs. control rats (two-way ANOVA). DC: diabetic control rats; DT: diabetic and trained; HC: health and control; HT: health and trained. Sol: soleus; EDL: extensor digitorum longus. Five rats in each group were utilized for this analysis. The analysis was performed in triplicate.
Supplier Page from Abcam for Anti-NRG1 antibody