Fig 1: Validation of expression of representative genes using immunohistochemistry. Overviews of DAB-immunohistochemical staining of OCM, PVALB, FBXO32, and SNCG and the representative images of the apical and basal regions indicated by the box in (A–D) were showed. Red asterisks indicate IHCs and black asterisks indicate OHCs in all panels. OCM (A) was highly expressed in OHCs while PVALB (B) and FBXO32 (C) expressed higher in basal than apical IHCs. No different expression of SNCG (D) has been found. (E) Comparison of IOD of representative genes between apical and basal IHCs. **p < 0.01. Scale bars: 100 µm for leftmost image of (A–D), 20 µm for right representative images of apical and basal regions of (A–D).
Fig 2: Validation of expression of representative genes using immunofluorescence in C57BL/6J mice. The basilar membrane was stained with antibodies against PVALB, OCM, FBXO32, and SNCG (red) and filamentous actin (green) was labeled with phalloidin. The labeling of PVALB (A) and FBXO32 (D) was intense in basal IHCs and weak in apical IHCs along the cochlear longitudinal axis. While OCM was highly expressed in OHCs and negatively expressed in IHCs (B). Selective expression deletion of Sncg (white triangles) was initially found in basal IHCs (C). Scale bar, 20 µm for all images in panels (A–D).
Fig 3: Validation of expression of representative genes using Immunofluorescence in BALBc mice. The basilar membrane was stained with antibodies against PVALB, FBXO32, and SNCG (red) and filamentous actin (green) was labeled with phalloidin. The labeling of PVALB (A) and FBXO32 (C) was higher in basal IHCs than that in apical IHCs while no selectively express deletion of SNCG (B) was observed in basal IHCs. Scale bar, 20 µm for all images in panels (A–C).
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