Fig 1: UC-MSC-CM is composed of cellular growth factors and possessed antioxidative ability. A: ELISA analysis of the concentration of various factors in the supernatant of MSCs-CM and fibroblast-CM; B: Representative images of Western blot analysis of SOD; C: Relative expression of SOD. Data represent the mean ± SD of 3 independent experiments. *P < 0.05 compared with Normal group, #P < 0.05 compared with MSCs-CM group
Fig 2: Physioxia stimulates antioxidant enzyme activity in keratinocyte cultures and in RHE. HaCaT and NHEK were submitted to physioxia (3% O2) or normoxia (18.6% O2) for 4 days. RHE were generated in physioxia (3% O2) or in normoxia (18.6% O2) for 15 days. Catalase and SOD enzymatic activities were evaluated in cells or RHE extracts. Data are presented by the relative enzymatic activities, normalized to protein amount, with mean ± SEM of at least 3 independent experiments. Statistical significance was determined using analysis of variance (ANOVA). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 between physioxia and normoxia.
Fig 3: Pomiferin treatment alleviated oxidative stress in lung tissues from mice with ARDS. (a–d) Representative western blots and semi-quantitative results of proteins of SOD1, SOD2, and GPX4 in lung tissues (n = 5). *P < 0.05 vs. control group, #P < 0.05 vs. ARDS group.
Fig 4: Physioxia modulates antioxidant enzyme amounts in RHE. RHE were generated in physioxia (3% O2) or in normoxia (18.6% O2) for 15 days. (a) Catalase, SOD1, and SOD2 were visualized by red immunofluorescence with a counterstain of nuclei with bisbenzimide. Scale bar: 50 µm. (b) Quantification of catalase, SOD1, and SOD2 from immunostainings. Data are represented by the relative protein amount with mean ± SEM of at least 3 independent experiments. Statistical significance was determined using analysis of variance (ANOVA). *p < 0.05 and **p < 0.01, between physioxia and normoxia.
Fig 5: Physioxia decreases antioxidant gene expression in keratinocyte cultures, HaCaT (a), NHEK (b), and RHE (c). HaCaT and NHEK were grown in physioxia (3% O2) or normoxia (18.6% O2) for 4 days. RHE were generated in physioxia or in normoxia for 15 days. Quantitative real-time PCR analyses were performed for mRNA expression of catalase, SOD1, SOD2, GPX1, and GPX4. The results were normalized to the three housekeeping genes GAPDH, B2M, and GUSB. Data are represented by the relative mRNA expression with mean ± SEM of at least 3 independent experiments. Statistical significance was determined using analysis of variance (ANOVA). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 between physioxia and normoxia.
Supplier Page from Abcam for Anti-Superoxide Dismutase 1 antibody