Fig 1: Artemin promotes proliferation of lung cancer cell lines. (a) NL9980 cells were transfected with pEGFP‐artemin or empty vector or untransfected for the indicated time. LTEP‐α‐2 cells were transfected with shARTN‐vector, negative control (NC) empty vector or untransfected. Cells were stained with propidium iodide and examined by fluorescence activated cell sorting. (b) Quantification of each cell phase is shown in histograms. Blank, NC, shARTN. Blank, Vector, pEGFP‐Artemin. (c) Effect of forced expression or knockdown of artemin on colony formation in soft agar. (d) Methyl thiazolyl tetrazolium assays in NL9980 and LTEP‐α‐2 cells transfected as indicated. A Blank, B NC, C shARTN, D Blank, E vector, F ARTN.