Anti Human Ago2, Monoclonal Antibody 015-22031 from FUJIFILM Wako Chemicals U.S.A. Corp

Product Specs
ItemAnti Human Ago2, Monoclonal Antibody
CompanyFUJIFILM Wako Chemicals U.S.A. Corp
Price Supplier Page
Catalog Number015-22031
ReactivityHuman
TargetAGO2
SpeciesHuman
ClonalityMonoclonal Antibody
Quantity50 µL (also 100 µL)
Conjugate/TagUnconjugated
FormatLiquid
ApplicationsWB, ICC, IP
Clone4G8
IsotypeIgG1
HostMouse

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FUJIFILM Wako Chemicals U.S.A. Corp

1600 Bellwood Road

Richmond, VA 23237-1326

United States

Phone: 804-271-7677
Fax: 804-271-7791

Email: wkuslabchem@fujifilm.com

Company Profile

Website: www.wakousa.com

(1) TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundanceNature communicationsSeptember 7, 2022Yang A, Bofill-De Ros X, Stanton R, Shao TJ, Villanueva P, Gu S.Magnetic Beads (Bio-Rad) plus 5 µg mouse anti-AGO1(2A7) (Wako, 015-22411) or anti-AGO2(4G8) (Wako, 015-22031) monoclonal antibody at 4 °C overnight with rotation. After washing 5 times with TBS buffer (50 mM Tris-HCl

(2) Development of Prodrug Type Circular siRNA for In Vivo Knockdown by Systemic AdministrationNucleic acid therapeuticsDecember 1, 2020Hagiwara K, Honma M, Harumoto T, Harada K, Sawada T, Yamamoto J, Shinohara F.analyzed by FLOWJO SOFTWARE (version 7.6.5). In vitro Ago2-associated uptake study Anti-Ago2 antibody (015-22031; Wako) or isotype control antibody (400102; BioLegend)-conjugated magnetic beads were prepared using

(3) AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2Nature communicationsJune 2, 2020Yang A, Shao TJ, Bofill-De Ros X, Lian C, Villanueva P, Dai L, Gu S.anti-Tubulin (1:3000, Sigma, #T9026), anti-AGO1 (1:500, Wako, #015-22411), and anti-AGO2 (1:500, Wako, #015-22031). The signals were developed with SuperSignal West Pico Chemiluminescent Substrate (Pierce) for strong

(4) In vivo rendezvous of small nucleic acid drugs with charge-matched block catiomers to target cancersNature communicationsApril 24, 2019Watanabe S, Hayashi K, Toh K, Kim HJ, Liu X, Chaya H, Fukushima S, Katsushima K, Kondo Y, Uchida S, et al.and 4 °C for 20 min, and the supernatant was collected. In parallel, anti-Argonaute 2 antibody (1 µg, 015-22031, Wako Pure Chemical Industries, Ltd., Osaka, Japan) was incubated overnight with 90 µL of Dynabeads® Protein G for immunoprecipitation

(5) ZEB1, ZEB2, and the miR-200 family form a counterregulatory network to regulate CD8(+) T cell fatesThe Journal of experimental medicineApril 2, 2018Guan T, Dominguez CX, Amezquita RA, Laidlaw BJ, Cheng J, Henao-Mejia J, Williams A, Flavell RA, Lu J, Kaech SM.RNA immunoprecipitation was performed with 10 µg pan-AGO antibody (MABE56; Millipore) and Ago2 antibody (015–22031; Wako Chemical), and quantitative RT-PCR was performed following a published protocol (Guo et al., 2015).

(6) FUS Regulates Activity of MicroRNA-Mediated Gene SilencingMolecular cellMarch 1, 2018Zhang T, Wu YC, Mullane P, Ji YJ, Liu H, He L, Arora A, Hwang HY, Alessi AF, Niaki AG, et al.,Santa Cruz,SC-47711 Ago2 (mouse monoclonal),Ago2 (mouse monoclonal),Ago2 (mouse monoclonal),WAKO chemicals,015-22031 Ago2 (rabbit polyclonal),Ago2 (rabbit polyclonal),Ago2 (rabbit polyclonal),Cell Signaling,C34C6 Ago2

(7) RNA interference–induced hepatotoxicity results from loss of the first synthesized isoform of microRNA-122 in miceNature MedicineMay 1, 2016Paul N. Valdmanis, Shuo Gu, Kirk Chu, Lan Jin, Feijie Zhang, Elizabeth M. Munding, Yue Zhang, Yong Huang, Huban Kutay, Kalpana Ghoshal, et al.21nt isoforms, leaving 66 microRNAs. For Ago2-immunoprecipitated fractions, a Wako antibody (catalog #015-22031) was used against Ago2. Liver powder samples were incubated with Anti-Ago2 overnight at 4° C, washed

(8) Engineering Duplex RNAs for Challenging Targets: Recognition of GGGGCC/CCCCGG Repeats at the ALS/FTD C9orf72 LocusChemistry & BiologyNovember 19, 2015Jiaxin Hu, Jing Liu, Liande Li, Keith T. Gagnon, David R. CoreyA/G agarose Plus beads and 300 uL of nuclear lysate were incubated with 5 ul of anti-AGO2 antibody (015–22031, Wako), or mouse anti-IgG antibody in 1×PBS (pH 7.4) at 4°C with gentle agita tion overnight. The beads

(9) Uncovering Direct Targets of MiR-19a Involved in Lung Cancer ProgressionPLOS ONESeptember 14, 2015Kumiko Yamamoto, Sachio Ito, Hiroko Hanafusa, Kenji Shimizu, Mamoru Ouchidain 3% BSA/PBS and then incubated for 4 h at room temperature with anti-AGO2 monoclonal antibody (No.016-20861, Wako, Osaka, Japan). The filters were washed with PBS/0.05% Tween-20 and then incubated with alkaline

(10) Characterization of the mammalian miRNA turnover landscapeNucleic Acids ResearchFebruary 27, 2015Yanwen Guo, Jun Liu, Sarah J. Elfenbein, Yinghong Ma, Mei Zhong, Caihong Qiu, Ye Ding, Jun LuImmunoprecipitation was performed with 10 μg of pan-AGO antibody (MABE56, Millipore), Ago2 antibody (015-22031, Wako Chemicals), Hsp90 antibody (sc-13119, Santa Cruz) and IgG antibody (12–371, Millipore) at 4°C

(11) Involvement of telomerase reverse transcriptase in heterochromatin maintenanceMolecular and cellular biologyMay 1, 2014Maida Y, Yasukawa M, Okamoto N, Ohka S, Kinoshita K, Totoki Y, Ito TK, Minamino T, Nakamura H, Yamaguchi S, et al.(07-442; Millipore), anti-histone H3 (06-755; Millipore), anti-DICER 13D6 (ab14601; Abcam), anti-AGO2 4G8 (015-22031; Wako), and anti-hTERT MAb (clone 10E9-2). The following antibodies were used for immunoprecipitation (IP

(12) 2'-OMe-phosphorodithioate-modified siRNAs show increased loading into the RISC complex and enhanced anti-tumour activityNature communicationsMarch 12, 2014Wu SY, Yang X, Gharpure KM, Hatakeyama H, Egli M, McGuire MH, Nagaraja AS, Miyake TM, Rupaimoole R, Pecot CV, et al.A5316), vinculin (1:2500, Sigma, V9131), GAPDH (1:5000, Sigma, G8795), Ago2 (1:100, Wako Chemicals, 015-22031), and GRAMD1B (1:500, Sigma, HPA008557) in 5% skim milk in TBS-T. Quantification was performed using

(13) The favorable IFNL3 genotype escapes mRNA decay mediated by AU-rich elements and hepatitis C virus–induced microRNAsNature ImmunologyJanuary 1, 2014Adelle P. McFarland et al.(GE Healthcare 17-0618-02) followed by overnight IP with 10 µg of control IgG or anti-Ago2 antibody (Wako 015-22031) conjugated to protein G beads. IPs were washed with polysome lysis buffers and aliquots were taken

(14) The flavonoid apigenin improves glucose tolerance through inhibition of microRNA maturation in miRNA103 transgenic miceScientific ReportsDecember 1, 2013Motoko Ohno et al.#SAB4200088; Dicer, #SAB4200087; TRBP2, #SAB4200111; β-actin, #A5441), Bethyl (KSRP, A302-021), Wako (Ago2, 015-22031), and Cell Signaling (Drosha, #D28B1; Phospho-Erk, #9101; Total Erk, #4695; myc-tag, #2276; Caveolin,

(15) RNA duplexes with abasic substitutions are potent and allele-selective inhibitors of huntingtin and ataxin-3 expressionNucleic Acids ResearchOctober 1, 2013Jing Liu, Hannah Pendergraff, K. Jayaprakash Narayanannair, Jeremy G. Lackey, Satya Kuchimanchi, Kallanthottathil G. Rajeev, Muthiah Manoharan, Jiaxin Hu, David R. Coreyand stored at −80°C. Protein A/G agarose Plus (60 µl) was incubated with 5 µl of anti-AGO2 antibody (015-22031, Wako) in phosphate buffered saline (pH 7.4) at 4°C with gentle agitation for 2 h (35). After two washes

(16) Off-Target Effect of Endogenous siRNA Derived from RMRP in Human CellsInternational Journal of Molecular SciencesApril 29, 2013Yoshiko Maida, Satoru Kyo, Timo Lassmann, Yoshihide Hayashizaki, Kenkichi MasutomiIgG (SC-2025, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or Anti Human Ago2, Monoclonal Antibody (015-22031, Wako Pure Chemical Industries, Osaka, Japan). RNA was isolated from the protein G beads using a miRNeasy Mini Kit

(17) Mechanism of allele-selective inhibition of huntingtin expression by duplex RNAs that target CAG repeats: function through the RNAi pathwayNucleic Acids ResearchDecember 1, 2012Jiaxin Hu, Jing Liu, Dongbo Yu, Yongjun Chu, David R. Coreyagarose Plus was incubated with 5 µl of anti-AGO1 (4B8, SAB4200084, Sigma), or anti-AGO2 antibody (015-22031, Wako), anti-GW182 antibody (A302-329A, Bethyl Laboratories) in 1× PBS (pH 7.4) at 4°C with gentle agitation

(18) Hypoxia Potentiates MicroRNA-Mediated Gene Silencing through Posttranslational Modification of Argonaute2Molecular and Cellular BiologyDecember 1, 2011Connie Wu, Jessica So, Brandi N. Davis-Dusenbery, Hank H. Qi, Donald B. Bloch, Yang Shi, Giorgio Lagna, Akiko Hatachemical inhibitors. Antibodies used in this study include anti-Ago1 (04-083; Millipore), anti-Ago2 (015-22031; Wako Chemicals), antiAgo3 (SAB4200112; Sigma), anti-Ago4 (05-967; Millipore), anti-Drosha (A301886A;

(19) Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinomaPloS oneJanuary 1, 2011Otsuka M, Takata A, Yoshikawa T, Kojima K, Kishikawa T, Shibata C, Takekawa M, Yoshida H, Omata M, Koike K.(SAB4200087; Sigma, St. Louis, MO), anti-TRBP2 (SAB4200087; Sigma), anti-β-actin (A5316; Sigma), anti-Ago2 (#015-22031; Wako, Osaka, Japan), anti-Gemin3 (DDX20) (SC-57007; Santa Cruz Biotechnology, Santa Cruz, CA), anti-Gemin4

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Anti Human Ago2, Monoclonal Antibody from FUJIFILM Wako Chemicals U.S.A. Corp

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FUJIFILM Wako Chemicals U.S.A. Corp

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Anti Human Ago2, Monoclonal Antibody from FUJIFILM Wako Chemicals U.S.A. Corp

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