Fig 1: Effects of BCRP silencing on excretion rates and intracellular levels of demethoxycurcumin-O-glucuronides in HeLa1A1 cells.(A) Effects of excreted demethoxycurcumin-O-glucuronides (G1) in extracellular medium; (B) Effects of excreted demethoxycurcumin-O-glucuronides (G2) in extracellular medium; (C) Effects of the efflux excretion rates of G1 and G2; (D) Effects of the intracellular G1 and G2 levels; (E) Effects of the fraction metabolized (fmet) of demethoxycurcumin; (F) Effects of gene silencing on the protein level of BCRP. The concentration of demethoxycurcumin was 4 μM. All experiments were performed in triplicate (n = 3). Data were presented as mean ± SD (n = 3). *, # p < 0.05, **, ## p < 0.01 and ***, ### p < 0.001 compared with that of control group of G1 or G2, respectively.