Fig 2: Immunofluorescence analysis of Adamdec1, Ednrb and Ptgs1 proteins from Zucker-lean+/fa and Zucker-Leprfa/fa rats both at baseline and after 30 days of placebo administration. Panels A–D show results for Adamdec1. Panels E–H show results for Ednrb. Panels I–L show results for Ptgs1. The green color corresponds to Alexa Fluor 488 dye. ZL, Zucker lean Lepr+/fa rats; ZO, Zucker-Leprfa/fa rats. n = 4 rats per group.

Fig 3: Immunofluorescence and western blot analysis of Adamdec1 and Ednrb proteins from Zucker-Leprfa/fa rats fed the placebo compared with Zucker-Leprfa/fa rats fed B. breve CNCM I-4035. In panels A and B, top graphs include 6–8 rats per group, and the lower parts show a representative crop blot. Hsp-70 was used as a loading control. *P < 0.05. In panel C, micrographs a,b (ZO placebo) and e,f (ZO + B. breve CNCM I-4035) show results for Adamdec1, and micrographs c,d (ZO placebo) and g,h (ZO + B. breve CNCM I-4036) show results for Ednrb. The green color corresponds to Alexa Fluor 488 dye. ZO, Zucker-Leprfa/fa rats.

Fig 4: Immunofluorescence and western blot analysis of Adamdec1 and Ednrb proteins from Zucker-Leprfa/fa rats fed the placebo compared with Zucker-Leprfa/fa rats fed L. paracasei CNCM I-4034. In panels A and B, top graphs include 7–8 rats per group, and the lower parts show a representative crop blot. Hsp-70 was used as a loading control. *p < 0.05. In panel C, micrographs a,b (ZO placebo), and e,f (ZO + L. paracasei CNCM I-4034) show results for Adamdec1, and micrographs c,d (ZO placebo), and g,h (ZO + L. paracasei CNCM I-4034) show results for Ednrb. The green color corresponds to Alexa Fluor 488 dye. ZO, Zucker-Leprfa/fa rats.

Fig 5: Western blot analysis of Adamdec1, Ednrb and Ptgs1 proteins from Zucker lean Lepr+/fa and Zucker-Leprfa/fa rats both at baseline and after 30 days of placebo administration. Panels A–C show results for Zucker lean Lepr+/fa rats at baseline compared with Zucker lean Lepr+/fa rats fed the placebo for 30 days. Panels D–F show results for Zucker-Leprfa/fa rats at baseline compared with Zucker-Leprfa/fa rats fed the placebo 30 days. The top graph included 7–8 rats per group, and the lower part shows a representative crop blot. Hsp-70 was used as a loading control. ZL, Zucker lean Lepr+/fa rats; ZO, Zucker-Leprfa/fa rats; *P < 0.05.