Fig 2: Interleukin-33 (IL-33) decreases bone marrow-derived monocytes (BMMs) osteoclastogenesis by inhibiting Notch1 activity. (A) Western blot analysis of Notch1 and Hes-1 expression in osteoclast progenitors incubated with CM-CRE in the presence of miR-34a-5p antagomir. (B–D) Quantification of Notch1, Jag1, and Hes-1 expression in osteoclast progenitors incubated with CM-CRE in the presence of miR-34a-5p antagomir. (E) Western blot analysis of Notch1 and Hes-1 expression in osteoclast progenitors induced by IL-33 in the presence of miR-34a-5p antagomir. (F–H) Quantification of Notch1, Jag1, and Hes-1 expression in osteoclast progenitors treated with IL-33 in the presence of miR-34a-5p antagomir. (I–K) BMMs osteoclastic formation and tartrate-resistant acid phosphatase (TRAP) activity assay after treatment with 20 ng/ml IL-33 in the presence of Jag-1. Red arrows indicated multinucleated osteoclasts. Scale bars represent 100 µm. (L–O) Quantification of Trap, Cathepsin K, Nfatc1, and C-fos expression in osteoclast progenitors treated with 20 ng/ml IL-33 in the presence of Jag-1. *P < 0.05, **P < 0.01, ***P < 0.001. P values were analyzed by one-way ANOVA.