Fig 1: Melanocytes exhibit two high molecular weight complexes containing WASH, only the higher one of which comigrates with strumpellin. (A) Western blot against antistrumpellin, anti-WASH, anti-SWIP, anti-Fam21, anti-CCDC53 and anti-WAFL with anti-a-tubulin loading control of control (CTRL) or strumpellin knockout (Str1 and Str2) immortal melanocyte line lysates. (B) Integrated density analysis of strumpellin, WASH, SWIP, CCDC53 and Fam21 expression in strumpellin knockout immortal melanocyte line lysates (Str1 and Str2) normalized against control (CTRL) immortal melanocyte line lysates (n = 3). (C) Schematic representation of the WASH complex (based on Jia et al., 2010). SWIP is green, Strumpellin (Str) is orange, Fam21 is red, WASH is shown with its VCA domain folded back into the complex in blue and a connecting line between the domains, and CCDC53 is purple. Protein sizes are not to scale. (D) Blue native PAGE Western blot showing antistrumpellin, anti-WASH, anti-SWIP, anti-Fam21, anti-CCDC53 and anti-WAFL in control (CTRL) or strumpellin knockout (Str1) immortal melanocyte line lysates.