beta Actin Antibody PA1-46296 from Thermo Fisher Scientific

Product Specs
Itembeta Actin Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPA1-46296
Brand/TrademarkABR (now Thermo Scientific Pierce Antibodies)
NCBI Gene AliasesActx, BRWS1, ACT-5, E430023M04Rik, PS1TP5BP1, beta-actin, Beta-actin
Quantity50 µL
Formattris citrate, tris phosphate with 0.09% sodium azide; pH 7-8
ApplicationsELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Proximity Ligation Assay (PLA), Western Blot
Targetbeta Actin
Conjugate/TagUnconjugated
ReactivityAvian, Bovine, Fish, Hamster, Human, Mouse, Non-human primate, Porcine, Rabbit, Rat
ClonalityPolyclonal
HostRabbit
NCBI Full Gene Nameactin beta, actin, beta
Concentration1 mg/mL
ImmunogenSynthetic peptide derived from the N-terminus of Human beta-Actin
IsotypeIgG
Storage Temperature4° C, do not freeze
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage4° C, do not freeze

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Protective effects of α-terpineol and Bacillus coagulans on intestinal function in weaned piglets infected with a recombinant Escherichia coli expressing heat-stable enterotoxin STaFrontiers in Veterinary ScienceFebruary 10, 2023Tao Wu, Qian Zhang, Haiwang Xu, Peng Li, Di Zhao, Lei Wang, Dan Yi, Yongqing HouAQP3 (ab125219, Abcam), Claudin (RF217968, Invitrogen), and Occludin (TC259714, Invitrogen), β-actin (PA1-46296, Invitrogen). Subsequently, appropriate HRP-conjugated secondary antibodies were used followed by incubation

(2) Epicatechin Prevents Cryocapacitation of Bovine Spermatozoa through Antioxidant Activity and Stabilization of Transmembrane Ion ChannelsInternational journal of molecular sciencesJanuary 28, 2023Baňas Š, Benko F, Ďuračka M, Lukáč N, Tvrdá E.USA" β-actin,beta Actin Polyclonal Antibody,rabbit,Polyclonal/IgG,1:1000,5% milk/TBS/0.1% Tween-20,PA1-46296,"Invitrogen, Waltham, MA, USA" Antibodies used in the Western blot analysis.

(3) Multifaceted Effects and Mechanisms of N-Acetylcysteine on Intestinal Injury in a Porcine Epidemic Diarrhea Virus-Infected Porcine ModelMolecular nutrition & food researchDecember 1, 2022Zhang Q, Li P, Li H, Yi D, Guo S, Wang L, Zhao D, Wang C, Wu T, Hou Y.

(4) The Effect of Heat Shock on Myogenic Differentiation of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal CellsCellsOctober 13, 2022Mikšiūnas R, Labeit S, Bironaitė D.Santa Cruz Biotechnology, Dallas, TX, USA), HSF1 (12972S, Cell Signaling Technology, Danvers, MA, USA), beta-actin (PA1-46296, Thermo Fisher Scientific, Waltham, MA, USA) were used to identify protein levels. Then the membranes

(5) Molecular neurobiological markers in the onset of sodium appetiteScientific reportsAugust 20, 2022Porcari CY, Cambiasso MJ, Mecawi AS, Caeiro XE, Antunes-Rodrigues J, Vivas LM, Godino A.scanner (LI-COR Biosciences). Membranes were re-probed with the rabbit polyclonal antibody, beta-Actin (PA1-46296) (Thermo Fisher Scientific Cat# PA1-46296, dilution 1:1500), to control protein loading. The membranes

(6) Poria Acid, Triterpenoids Extracted from Poria cocos, Inhibits the Invasion and Metastasis of Gastric Cancer CellsMolecules (Basel, Switzerland)June 6, 2022Wang H, Luo Y, Chu Z, Ni T, Ou S, Dai X, Zhang X, Liu Y.(Thermo Fisher Scientific, Waltham, MA, USA, Cat. no. PA5-85197, PA5-16509, PA5-32178, PA5-29570, MA5-16409, PA146296); TIMP-1 (Abcam, Cambridge, UK, Cat. no. ab211926). 2.3. Cell Culture The AGS and MKN-28 human gastric cancer

(7) Cell clusters containing intestinal stem cells line, the zebrafish intestine intervillus pocketiScienceMay 20, 2022Tavakoli S, Zhu S, Matsudaira P.were used as follows: Rabbit anti-PRMT1 antibody (GTX128199) and anti-β-actin antibody (ThermoFisher PA1-46296). Secondary antibodies were from Tyramide-Alexa Fluor 647 (Invitrogen T20916) and Alexa Fluor 555 (Invitrogen

(8) Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In VitroMolecules (Basel, Switzerland)February 2, 2022Chu Z, Luo Y, Ni T, Zhu M, Feng X, Liu Y, Wang H.PA5-85197, Cat. no. PA5-16509, Cat. no. PA5-32178, Cat. no. PA5-29570, Cat. no. MA5-16409, Cat. no. PA146296); TIMP1, F-actin, GAPDH (Abcam, Cambridge, UK, Cat. no. ab211926, Cat. no. ab130935, Cat. no. ab8245);

(9) Monolaurin Confers a Protective Effect Against Porcine Epidemic Diarrhea Virus Infection in Piglets by Regulating the Interferon PathwayFrontiers in ImmunologyJanuary 13, 2022Qian Zhang, Dan Yi, Changzheng Ji, Tao Wu, Manli Wang, Shuangshuang Guo, Lei Wang, Di Zhao, Yongqing Houanti-DDX58 (4200, Cell Signaling Technology)/1:1000, anti-ISG15 (ab233071, Abcam)/1:1000, and anti-β-Actin (PA1-46296, Invitrogen)/1:4000. Results Growth Performance Prior to PEDV infection, oral administration of

(10) Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1Frontiers in oncologyJanuary 1, 2022Guan H, Mao L, Wang J, Wang S, Yang S, Wu H, Sun W, Chen Z, Chen M.(SAB1407636), anti-CHD1 (ZRB1692) and anti-PSMD8 (SAB1406325) provided by Sigma-Aldrich, and anti-β-actin (PA1-46296), anti-CD63 (MA1-19281), anti-TSG101 (1062BD), anti-calnexin (PA5-34665), anti-HDAC1 (49-1025), anti-RAN

(11) Immune Synapse Residency of Orai1 Alters Ca(2+) Response of T CellsInternational journal of molecular sciencesOctober 26, 2021Voros O, Panyi G, Hajdu P.Sigma-Aldrich, Budapest, Hungary) or mouse anti-SAP97 (BioLegend, MMS-5187) and rabbit anti-actin (PA1-46296, Thermo Fisher Scientific Inc., Budapest, Hungary) primary or and anti-mouse IgG HRP-linked or anti-rabbit IgG HRP-linked

(12) CoQ10 augments candesartan protective effect against tourniquet-induced hind limb ischemia-reperfusion: Involvement of non-classical RAS and ROS pathwaysSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical SocietyJuly 1, 2021Awad AS, Nour El-Din M, Kamel R.dilution) for p38 MAPK (Cat. No. PA5-17713), phospho-p38 MAPK (Cat. No. 44-684G) and β-actin (Cat. No. PA1-46296) (Thermo Scientific, Rockford, Illinois, USA). After washing, membranes were incubated at 37 °C for

(13) Reduction of Renal Preservation/Reperfusion Injury by Controlled Hyperthermia During Ex Vivo Machine PerfusionClinical and translational scienceMarch 1, 2021Minor T, von Horn C.normalized against the expression of total ERK (Thermo Fisher, #MA5‐15174) or ß‐actin (Thermo Fisher, #PA1‐46296). Statistics All values are expressed as means ±SD for n = 7 animals per group and differences between

(14) Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer EffectorsMolecules (Basel, Switzerland)November 9, 2020Choi JW, Lim S, Kang JH, Hwang SH, Hwang KC, Kim SW, Lee S.associated with cytotoxicity of NKL-derived EVs against cancer, neutralizing antibodies of β-actin (PA1-46296, Thermo Fisher), fibrinogen (ab34269, Abcam, Cambridge, UK), and isotype control (rabbit IgG, polyclonal;

(15) Neuroprotective role of delta opioid receptors in hypoxic preconditioningTURKISH JOURNAL OF MEDICAL SCIENCESOctober 24, 2019Şevin GÜNEY, Sibel DİNÇER, Güleser GÖKTAŞ, Gülnur TAKE-KAPLANOĞLU(Cell Signaling Technology, Bcl-2, #2870, 1:3000; cyt-c #4280, 1:1000; beta actin, Thermo Scientific PA1-46296, 1:2000) Beta actin was used as the loading control. After washing 3 times in TBS containing 0.1% Tween-20,

(16) M1-Polarized Macrophages Promote Self-Renewing Phenotype of Hepatic Progenitor Cells with Jagged1-Notch Signalling Involved: Relevance in Primary Sclerosing CholangitisJournal of immunology researchJanuary 1, 2018Li H, Sun S, Lei Q, Lei P, Cai X, Wan C, Shen G.No. 141703 Invitrogen Catalog No. MA5-15012 Invitrogen Catalog No. PA5-23283 Invitrogen Catalog No. PA1-46296 Abcam Catalog No. ab175471 1 : 100 1 : 100 1 : 100 1 : 100 1 : 100 1 : 100 1 : 100 1 : 100 1 : 100 1 : 500

(17) miR-93‑mediated collagen expression in stress urinary incontinence via calpain-2Molecular medicine reportsJanuary 1, 2018Yang SJ, Wang J, Xu J, Bai Y, Guo ZJ.Inc.), anti‑MMP1 (cat. no. MA1‑771; Invitrogen; Thermo Fisher Scientific, Inc.), anti‑β‑actin (cat. no. PA1‑46296; Invitrogen; Thermo Fisher Scientific, Inc.) and anti‑collagen I (cat. no. PA1‑26204; Invitrogen; Thermo

(18) GFAP expression is influenced by astrocytoma grade and rs2070935 polymorphismJournal of CancerJanuary 1, 2018Sereika M, Urbanaviciute R, Tamasauskas A, Skiriute D, Vaitkiene P.with a flatbed scanner to produce digital images. Afterwards, polyclonal anti-Beta-actin (Catalog#: PA1-46296, Thermo Fisher Scientific) and anti‑rabbit (Catalog#: 31460, Thermo Fisher Scientific) antibodies were

(19) Knockdown of COBRA1 decreases the proliferation and migration of hepatocellular carcinoma cellsOncology reportsMarch 1, 2017El Zeneini E, Kamel S, El-Meteini M, Amleh A.knockdown analysis) (1:1,000 in 5% non-fat dry milk in 0.01% PBST), rabbit polyclonal anti-B-ACTIN (Pierce, PA1-46296) or mouse monoclonal anti-B-TUBULIN (Sigma, T7816) (1:20,000 in 5% non-fat dry milk in 0.01% PBST) at

(20) Gestation age dependent transfer of human immunoglobulins across placenta in timed-pregnant guinea pigsPlacentaDecember 1, 2015Xu Y, Ma L, Norton MG, Stuart C, Zhao Z, Toibero D, Dahlen S, Zhong L, Zhang P, Struble EB.antibodies specific for the FcRn alpha chain (anti-human FCGRT, Proteintech, 1:200) and anti-β–actin (PA1-46296, Thermo Scientific, 1:2000), then goat anti-rabbit antibodies (1:2000) and imaged as before [20]. For

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beta Actin Antibody from Thermo Fisher Scientific

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