Fig 2: Mice expressing apoA-V Q252X have elevated plasma TG. Apoa5 KO mice were injected with 3E11 vg Null, WT or Q252X hAPOA5 AAV. A. Relative fold expression APOA5 in whole cell liver lysate (N=4-5). B. Relative fold expression APOA5 in liver nuclear mRNA (N=3-5). C. Relative abundance of APOA5 over time in CHO cells, transiently transfected to express WT or Q252X APOA5 then exposed to actinomycin-D. (N=3). D. Plasma TG levels at baseline and 4 weeks after AAV administration. Results are expressed as percent of baseline levels (N=5-11). E. TG content and F. TC content of plasma lipoproteins, separated by fast protein liquid chromatography (FPLC). Plasma samples were pooled by experimental group prior to fractionation. Fractions corresponding to the elution range of VLDL, LDL and HDL particles are indicated. G. ApoA-V content of plasma lipoproteins, separated by fast protein liquid chromatography (FPLC). Indicated FPLC fractions were pooled and immunoblotted to detect WT and Q252X apoA-V. A-D. Mean with SEM (brackets). E, F. Points indicate single value. Unpaired 2-tailed t-test. p<0.05 = *, p<0.001 = *** vs. Null. p<0.05 = $, p<0.01 = $$, p<0.001 = $$$, p<0.0001 = $$$$ vs. WT. apoA-V, apolipoprotein A5; CHO, Chinese Hamster Ovary; FPLC, fast protein liquid chromatography; HDL, high-density lipoprotein; KO, knockout; LDL, low-density lipoprotein; TC, total cholesterol; TG, triglyceride; VLDL, very low-density lipoprotein.