Fig 2: Increased H3K9me2 in the cerebellar granule cell layer of Kdm3b+/- mice. a Microdissection of cerebellar vermis to separate granule-cell-enriched (GCE) and Purkinje-cell-enriched (PCE) samples. b, c Layer-specific quantification of H3K9me2 level in GCE and PCE samples from Kdm3b+/+ and Kdm3b+/- mice. N = 3 mice per group. An independent t-test was performed to test statistical significance between two groups. **p < 0.01. n.s indicates p > 0.05

Fig 3: Knockout of Kdm3b in male mice altered their sexual behaviors. A & B. The average latency time that 8-week-old WT (+/+, n = 10), heterozygous (+/-, n = 9) and Kdm3bKO (-/-, n = 12) male mice took to mount the stimulus females, and the total number of mounts that these male mice carried out during the testing period. * & **, p < 0.05 and p < 0.01 by One-Way ANOVA. C & D. The average intromission latency time that 8-week-old WT (n = 10), heterozygous (n = 8) and Kdm3bKO (n = 11) male mice achieved their first intromission after the stimulus females were introduced, and the number of successful intromissions that these mice carried out during the testing period. **, p < 0.01 by One-Way ANOVA. E. The average latency time that 8-week-old WT (n = 10), heterozygous (n = 8) and Kdm3bKO (n = 11) male mice took to ejaculate after the stimulus females were introduced. **, p < 0.01 by One-Way ANOVA.

Fig 4: Kdm3b is required for maintaining normal levels of 17ß-estradiol. (A). The concentrations of 17ß-estradiol in the serum samples prepared from WT and Kdm3bKO (KO) mice on day 8 of pregnancy (n=6), at 40 hours after PMSG injection (n=6), and at 24 hours after hCG injection (n=3). (B). The concentrations of progesterone in the serum samples of WT and KO mice on day 8 pregnancy (n=6) and at 24 hours after hCG injection (n=3). (C). The relative expression levels of Cyp19a1 mRNA in the ovaries of untreated WT and KO mice (n=5).

Fig 5: Knockout of Kdm3b impaired the motivational components of the male sexual behaviors. The average time that WT (+/+), n = 10), heterozygous (+/-, n = 9) and Kdm3bKO (-/-, n = 12) male mice spent on investigating the clean bedding materials (Panel A), the bedding materials with male odor (Panels B), the bedding materials with female odor (Panel C), and the Oreo cookie (Panel D). **, p < 0.01 by One-Way ANOVA.